|
| Status |
Public on Aug 11, 2014 |
| Title |
Partial phenotypic rescue of the Sesb1 mitochondrial ANT1 disease model in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Study of gene expression patterns of Drosophila melanogaster Sesb1 mutants compared to wild type
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| |
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| Overall design |
Inbreeding under stressful conditions inevitably results in the selection of compensatory alleles of many genes. In order to avoid such issues, and thus determine the global effects on gene expression of the Sesb1 mutation in a truly unselected and 'wild-type' background, we outbred Sesb1 over more than 10 generations by back-crossing to each of two commonly used wild-type strains, Canton S and Oregon R. Subsequent to this backcrossing, Sesb1 was maintained in each background using a balancer chromosome. These stocks were then used to generate a Sesb1 mutant F1 generation, by crossing virgin Canton S Sesb1. homozygous mutant females with Oregon R Sesb1 males. For comparison, we generated otherwise isogenic wild-type F1 progeny by crossing virgin Canton S wild-type females with Oregon R wild-type males. Progeny was collected on the day of eclosion and aged for one day after which the RNA was isolated with Trizol. The quality of the RNA was analyzed with a spectrophotometer.
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| Contributor(s) |
Vartiainen S, Chen S, Tuomela T, Luoto K, O'Dell K, Jacobs H |
| Citation(s) |
24812436 |
| Submission date |
Feb 19, 2014 |
| Last update date |
May 04, 2018 |
| Contact name |
Suvi Vartiainen |
| Organization name |
University of Tampere
|
| Department |
Institute of Biomedical Technology
|
| Street address |
Biokatu 6
|
| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
|
| Samples (14)
|
| GSM1331249 |
Wild type male flies biological replicate 1 |
| GSM1331250 |
Wild type male flies biological replicate 2 |
| GSM1331251 |
Wild type male flies biological replicate 3 |
| GSM1331252 |
Wild type female flies biological replicate 1 |
| GSM1331253 |
Wild type female flies biological replicate 2 |
| GSM1331254 |
Wild type female flies biological replicate 3 |
| GSM1331255 |
Sesb1 mutant male flies biological replicate 1 |
| GSM1331256 |
Sesb1 mutant male flies biological replicate 2 |
| GSM1331257 |
Sesb1 mutant male flies biological replicate 3 |
| GSM1331258 |
Sesb1 mutant male flies biological replicate 4 |
| GSM1331259 |
Sesb1 mutant female flies biological replicate 1 |
| GSM1331260 |
Sesb1 mutant female flies biological replicate 2 |
| GSM1331261 |
Sesb1 mutant female flies biological replicate 3 |
| GSM1331262 |
Sesb1 mutant female flies biological replicate 4 |
|
| Relations |
| BioProject |
PRJNA238831 |
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