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| Status |
Public on Sep 22, 2015 |
| Title |
Expression data in dSTING knockdown and control sibling flies after mock or pathogenic infection |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Parental flies (y1v1;P{TRiP.JF01138}attP2 and y1w*;P{Act5C-GAL4}25FO1/CyO,y+) were mated and 4-7 day old flies were used for all subsequent experiments. dSTING RNAi flies expressed the dsRNA hairpin targeting dSTING and had straight wings, while sibling flies not expressing dSTING dsRNA had curly wings.
Pathogens used for inoculation were Listeria monocytogenes, Drosophila C Virus (DCV), and Insect Iridescent virus 6 (IIV6), at the following concentrations: 1e7 CFU/ml, 5e7 TCID50/ml, 5e6 TCID50/ml. Mock inoculation was performed with PBS. Whole flies were homogenized 24 or 72 h post-infection.
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| Overall design |
Determining the effect of genotype on gene expression profiles following pathogenic infection was the overall goal of this project.
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| Contributor(s) |
Goodman AG, Barber GN |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 10, 2014 |
| Last update date |
May 04, 2018 |
| Contact name |
Glen N. Barber |
| Organization name |
University of Miami
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| Department |
Cell Biology
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| Street address |
1550 NW 10th Ave
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| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (40)
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| GSM1324568 |
mock infected knockdown flies, 24hpi, rep1 |
| GSM1324569 |
mock infected knockdown flies, 24hpi, rep2 |
| GSM1324570 |
mock infected knockdown flies, 24hpi, rep3 |
| GSM1324571 |
mock infected knockdown flies, 24hpi, rep4 |
| GSM1324572 |
mock infected control flies, 24hpi, rep1 |
| GSM1324573 |
mock infected control flies, 24hpi, rep2 |
| GSM1324574 |
mock infected control flies, 24hpi, rep3 |
| GSM1324575 |
mock infected control flies, 24hpi, rep4 |
| GSM1324576 |
mock infected knockdown flies, 72hpi, rep1 |
| GSM1324577 |
mock infected knockdown flies, 72hpi, rep2 |
| GSM1324578 |
mock infected knockdown flies, 72hpi, rep3 |
| GSM1324579 |
mock infected knockdown flies, 72hpi, rep4 |
| GSM1324580 |
mock infected control flies, 72hpi, rep1 |
| GSM1324581 |
mock infected control flies, 72hpi, rep2 |
| GSM1324582 |
mock infected control flies, 72hpi, rep3 |
| GSM1324583 |
mock infected control flies, 72hpi, rep4 |
| GSM1324584 |
IIV6 infected knockdown flies, 24hpi, rep1 |
| GSM1324585 |
IIV6 infected knockdown flies, 24hpi, rep2 |
| GSM1324586 |
IIV6 infected control flies, 24hpi, rep1 |
| GSM1324587 |
IIV6 infected control flies, 24hpi, rep2 |
| GSM1324588 |
IIV6 infected knockdown flies, 72hpi, rep1 |
| GSM1324589 |
IIV6 infected knockdown flies, 72hpi, rep2 |
| GSM1324590 |
IIV6 infected control flies, 72hpi, rep1 |
| GSM1324591 |
IIV6 infected control flies, 72hpi, rep2 |
| GSM1324592 |
DCV infected knockdown flies, 24hpi, rep1 |
| GSM1324593 |
DCV infected knockdown flies, 24hpi, rep2 |
| GSM1324594 |
DCV infected control flies, 24hpi, rep1 |
| GSM1324595 |
DCV infected control flies, 24hpi, rep2 |
| GSM1324596 |
DCV infected knockdown flies, 72hpi, rep1 |
| GSM1324597 |
DCV infected knockdown flies, 72hpi, rep2 |
| GSM1324598 |
DCV infected control flies, 72hpi, rep1 |
| GSM1324599 |
DCV infected control flies, 72hpi, rep2 |
| GSM1324600 |
listeria infected knockdown flies, 24hpi, rep1 |
| GSM1324601 |
listeria infected knockdown flies, 24hpi, rep2 |
| GSM1324602 |
listeria infected control flies, 24hpi, rep1 |
| GSM1324603 |
listeria infected control flies, 24hpi, rep2 |
| GSM1324604 |
listeria infected knockdown flies, 72hpi, rep1 |
| GSM1324605 |
listeria infected knockdown flies, 72hpi, rep2 |
| GSM1324606 |
listeria infected control flies, 72hpi, rep1 |
| GSM1324607 |
listeria infected control flies, 72hpi, rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE54834 |
Expression data in dSTING knockdown S2 cells and whole flies after mock or pathogenic infection |
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| Relations |
| BioProject |
PRJNA237780 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE54833_RAW.tar |
79.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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