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| Status |
Public on Jun 30, 2014 |
| Title |
Polysomes from DENR knockdown cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
The aim was to identify transcripts that are poorly translated upon knockdown of DENR. Lysates from control (GFP) and DENR knockdown S2 cells were run on polysome gradients.
RNA from the (80S peak + polysome peaks) was isolated and analyzed by microarrays. In parallel, total RNA from the cells was also profiled as a normalization control and profiled.
The 'translation score' of translated mRNA to total RNA was then calculated for control and DENR knockdown cells.
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| Overall design |
Two biological replicates for DENR knockdown and GFP knockdown were performed. For each sample, (80S+polysomes) and total RNA were profiled, leading to 8 samples in total.
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| Contributor(s) |
Schleich S, Strassburger K, Janiesch PC, Koledachkina T, Miller KK, Haneke K, Cheng Y, Kuechler K, Stoecklin G, Duncan KE, Teleman AA |
| Citation(s) |
25043021 |
| Submission date |
Feb 03, 2014 |
| Last update date |
May 04, 2018 |
| Contact name |
Aurelio A Teleman |
| Organization name |
German Cancer Research Center (DKFZ)
|
| Street address |
Im Neuenheimer Feld 580
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA237227 |
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