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| Status |
Public on Jan 31, 2014 |
| Title |
Lipopolysaccharide modulates rhinovirus-induced chemokine secretion in monocytes and macrophages. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Rhinovirus (RV) infection has been shown to exacerbate asthma. Understanding of the underlying mechanisms by which RV infections provoke airway inflammation and remodeling in asthma may offer significant insight into asthma pathogenesis and exacerbations as well as offer opportunities for improved disease management. Expression analysis of RV A16-treated primary peripheral blood monocytes from human donors was performed to determine what pattern of gene expression is associated with RV infection of monocytes in vitro.
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| Overall design |
Peripheral blood monocytes were isolated from 5 adults. Following overnight incubation in RPMI supplemented with 10% FBS, the monocytes were stimulated with purified major group RV A16 at multiplicity of infection of 10 plaque forming units (pfu) per cell or with growth media alone (mock). Cells were lysed 4 hours after treatment and isolated total RNAs were pooled per treatment and analyzed using the Affymetrix GeneChip HuGeneFL Array (Hu6800, Affymetrix, Santa Clara, CA).
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| Contributor(s) |
Bates M, Hall DJ, Karta MR, Bertics PJ |
| Citation(s) |
24498897 |
| Submission date |
Jan 30, 2014 |
| Last update date |
Jul 08, 2016 |
| Contact name |
Maya R Karta |
| Organization name |
University Wisconsin-Madison
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| Department |
Biomolecular Chemistry
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| Lab |
Paul J. Bertics
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| Street address |
1135 Biochemistry, 420 Henry Mall
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL80 |
[Hu6800] Affymetrix Human Full Length HuGeneFL Array |
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| Samples (2) |
| GSM1318806 |
mock-infected monocytes, 4 h after treatment |
| GSM1318807 |
RV A16-infected monocytes, 4 h after treatment |
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| Relations |
| BioProject |
PRJNA237025 |
