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| Status |
Public on Jan 18, 2014 |
| Title |
Expression data from mouse limb tendon cells during development. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We have undertaken a screen of mouse limb tendon cells in order to identify molecular pathways involved in tendon development. Mouse limb tendon cells were isolated based on Scleraxis (Scx) expression at different stages of development: E11.5, E12.5 and E14.5
Microarray comparisons were carried out between tendon progenitor and differentiated stages.
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| Overall design |
Forelimbs from E11.5, E12.5 and E14.5 Scx-GFP embryos were collected and dissociated with trypsin to obtain cell suspensions. Scx-positive tendon cells were isolated by FACS. RNA was extracted and Fragmented biotin-labelled cRNA samples were hybridized on Affymetrix Gene Chip Mouse Genome 430 2.0 arrays.
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| |
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| Contributor(s) |
Duprez D, Croze S, Lachuer J, Nazaret N |
| Citation(s) |
25249460 |
| Submission date |
Jan 17, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Delphine Duprez |
| E-mail(s) |
delphine.duprez@upmc.fr
|
| Phone |
+33 1 44 27 27 53
|
| Organization name |
CNRS/UPMC
|
| Department |
UMR 7622
|
| Lab |
Developmental Biology
|
| Street address |
9, Quai Saint Bernard
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA235887 |
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