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| Status |
Public on Oct 13, 2014 |
| Title |
The effects of muscular dystrophy, exercise and sActRIIB-Fc on molecular signature of skeletal muscle in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To study the combined effect of myostatin/activin inhibition and exercise on muscle mass and pathophysiology, young mdx mice, a model for Duchenne Muscular Dystrophy, were injected with soluble activin receptor-Fc (sActRIIB-Fc) or placebo (PBS) 1x/week for a 7-week period, in combination with or without voluntary running. C57Bl/10ScSnJ mice injected with PBS acted as wildtype controls. Microarray expression analysis from skeletal muscle was performed using m. gastrocnemies as the sample.
We found thatexercise or a combination of exercise and sActRIIB-Fc treatment is more effective in correcting gene expression profiles of dystrophic muscles than the sActRIIB-Fc treatment alone. We also identified several pathways and proteins that were affected by exercise and sActRIIB-Fc together or independently.
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| Overall design |
Total RNA obtained from gastrocnemius muscle of mdx mice divided into four groups:
1) control (injected with PBS, n=5), 2) runners (voluntary wheel running for 7 weeks, injected with PBS, n=5), 3) sActRIIB-Fc -treated (n=5), and 4) runners with sActRIIB-Fc-treatment (n=5).
sActRIIB-Fc or PBS was injected intraperitoneally once a week with a 5-mg/kg dose of sActRIIB-Fc. In addition, wildtype mice served as healthy controls (n=4).
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| Contributor(s) |
Hulmi JJ, Kainulainen H, Papaioannou KG, Silvennoinen M, Saarela J, Oliveira BM, Nyqvist M, Pasternack A, ’t Hoen PA, Kujala UM, Ritvos O, Autio R |
| Citation(s) |
25304272 |
| Submission date |
Nov 26, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Juha J Hulmi |
| E-mail(s) |
juha.hulmi@jyu.fi
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| Organization name |
University of Jyväskylä
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| Department |
Biology of Physical Activity
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| Street address |
Rautpohjankatu 8a Viv
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| City |
Jyväskylä |
| ZIP/Postal code |
40014 |
| Country |
Finland |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (24)
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| GSM1275712 |
Mdx skeletal muscle, PBS+running for 7 weeks rep 1 |
| GSM1275713 |
Mdx skeletal muscle, PBS+running for 7 weeks rep 2 |
| GSM1275714 |
Mdx skeletal muscle, PBS+running for 7 weeks rep 3 |
| GSM1275715 |
Mdx skeletal muscle, PBS+running for 7 weeks rep 4 |
| GSM1275716 |
Mdx skeletal muscle, PBS+running for 7 weeks rep 5 |
| GSM1275717 |
Mdx skeletal muscle, sActRIIB-Fc + running for 7 weeks rep 1 |
| GSM1275718 |
Mdx skeletal muscle, sActRIIB-Fc + running for 7 weeks rep 2 |
| GSM1275719 |
Mdx skeletal muscle, sActRIIB-Fc + running for 7 weeks rep 3 |
| GSM1275720 |
Mdx skeletal muscle, sActRIIB-Fc + running for 7 weeks rep 4 |
| GSM1275721 |
Mdx skeletal muscle, sActRIIB-Fc + running for 7 weeks rep 5 |
| GSM1275722 |
Wildtype skeletal muscle, no treatment rep 1 |
| GSM1275723 |
Wildtype skeletal muscle, no treatment rep 2 |
| GSM1275724 |
Wildtype skeletal muscle, no treatment rep 3 |
| GSM1275725 |
Wildtype skeletal muscle, no treatment rep 4 |
| GSM1275726 |
Mdx skeletal muscle, PBS treatment for 7 weeks rep 1 |
| GSM1275727 |
Mdx skeletal muscle, PBS treatment for 7 weeks rep 2 |
| GSM1275728 |
Mdx skeletal muscle, PBS treatment for 7 weeks rep 3 |
| GSM1275729 |
Mdx skeletal muscle, PBS treatment for 7 weeks rep 4 |
| GSM1275730 |
Mdx skeletal muscle, PBS treatment for 7 weeks rep 5 |
| GSM1275731 |
Mdx skeletal muscle, sActRIIB-Fc treatment for 7 weeks rep 1 |
| GSM1275732 |
Mdx skeletal muscle, sActRIIB-Fc treatment for 7 weeks rep 2 |
| GSM1275733 |
Mdx skeletal muscle, sActRIIB-Fc treatment for 7 weeks rep 3 |
| GSM1275734 |
Mdx skeletal muscle, sActRIIB-Fc treatment for 7 weeks rep 4 |
| GSM1275735 |
Mdx skeletal muscle, sActRIIB-Fc treatment for 7 weeks rep 5 |
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| Relations |
| BioProject |
PRJNA229986 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52766_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE52766_non_normalized.txt.gz |
3.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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