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| Status |
Public on Nov 20, 2013 |
| Title |
Transcriptional program for induced delta cell on day10 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Direct lineage conversion of adult cells is a promising approach for regenerative medicine. A major challenge of lineage conversion is to generate specific subtypes of cells, closely related cells with distinct properties. The pancreatic islets contain three major hormone-secreting endocrine subtypes: insulin+ β-cells, glucagon+ α-cells, and somatostatin+ δ-cells. We previously reported that a combination of three transcription factors, Ngn3, Mafa, and Pdx1, directly reprogram pancreatic acinar cells to β-cells. We now show that acinar cells can be converted to δ-like and α-like cells by Ngn3 and Ngn3+Mafa respectively. Thus, three major islet endocrine subtypes can be derived by acinar reprogramming. Ngn3 promotes establishment of a generic endocrine state in acinar cells at the onset of reprogramming in addition to promoting δ-specification. Mafa and Pdx1 suppress δ-specification in α- and β-cell formation. These studies identify a set of defined factors whose combinatorial actions reprogram acinar cells to distinct islet endocrine subtypes in vivo.
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| Overall design |
induced beta cells samples at day 10 collected for the microarray
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| Contributor(s) |
Li W, Zhou Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 19, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Weida Li |
| E-mail(s) |
weidali@mcb.harvard.edu
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| Phone |
6174961687
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| Organization name |
Harvard University
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| Street address |
7 Divninity Avenue
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA229154 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52522_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE52522_non-normalized.txt.gz |
609.9 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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