 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 11, 2014 |
| Title |
Mapping of a QTL for airway hyperresponsiveness on mouse chromosome 12 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The BcA86 strain is a unique recombinant congenic strain created from parental strains A/J and C57BL/6J. Naive mice from the BcA86 strain have a lung responsiveness phenotype resembling mice from airway hyperresponsive strain A/J. However, majority of the BcA86 genome is from the hyporesponsive strain C57BL/6J. Our goal was to identify the genomic regions that are associated with this BcA86 phenotype. Using F2 mice generated from BcA86 backcrossed to C57BL/6J, we identified a QTL for airway hyperresponsiveness on mouse chromosome 12. We validated the importance of mouse chromosome 12 in airway responsiveness using a chromosome 12 substitution strain (CSS12) which contains A/J chromosome 12 on a C57BL/6J background. The CSS12 strain also had a lung responsiveness phenotype similar to A/J. We selected genes within our QTL as candidates for airway hyperresponsiveness if they contained a deleterious coding variant (based on PROVEAN analysis) or if they were differently expressed between hyperresponsive (A/J, BcA86, CSS12) and hyporesponsive (C57BL/6J) strains.
|
| |
|
| Overall design |
Based on microarray analysis followed by validation with RT-QPCR, genes within the QTL that are differently expressed between hyperresponsive strains (CSS12 and BcA86) and hyporesponsive strain (C57Bl/6J) were selected as candidates for airway hyperresponsiveness.
|
| |
|
| Contributor(s) |
Kanagaratham C, Sladek R, Vidal SM, Radzioch D |
| Citation(s) |
25111050 |
| Submission date |
Nov 13, 2013 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Cynthia Kanagaratham |
| E-mail(s) |
cynthia.kanagaratham@mail.mcgill.ca
|
| Organization name |
McGill University
|
| Department |
Human Genetics
|
| Lab |
Danuta Radzioch
|
| Street address |
1650 Cedar Ave.
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G 1A4 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA227474 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52356_RAW.tar |
83.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...