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| Status |
Public on Dec 31, 2014 |
| Title |
Dynamic Changes in E-protein Activity are Essential for Treg cell Development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To gain a molecular view of E-proteins with respect to the development of Foxp3+ T cells, we perform microarray studies that would identify transcription factors that are up-regulated as E-proteins levels fall and and Foxp3 expression rises. We hypothesize that such transcription factors activate the synthesis of key proteins necessary for the development of Foxp3+ cells in the thymus (or in the periphery). Among the possible proteins (negatively regulated by E-protein are those involved in IL-2 signaling, since the latter has been shown to be critical for the development of Foxp3+ cells in the thymus. In the planned studies we will extract mRNA from sorted Foxp3+GFP+ thymocytes from wild type (WT) mice and similar cells from E-protein deficient thymocytes. However, since Foxp3+ thymocytes in WT mice already have reduced E-protein levels, differences between thymocytes from these mice and E-protein deficient mice might be minimal. We therefore will also extract mRNA from Foxp3-negative thymocytes from WT mice whose E-proteins levels have not fallen sufficient low to allow Foxp3 expression and Foxp3-negative thymocytes from E-protein deficient mice whose E-proteins are necessarily low but have not expressed Foxp3 for unrelated reasons.
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| Overall design |
Isolate mRNA from sorted Foxp3+ and Foxp3- thymocytes from either wild type (WT) mice and from E-protein deficient (E2A and HEB knockout) thymocytes; 2 replicates of each of the 4 conditions
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| Contributor(s) |
Gao P, Yang JZ, Fuss I, Zhang Q, Myers TG, Gardina PJ, Zhang F, Strober W |
| Citation(s) |
25488982 |
| Submission date |
Oct 24, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Timothy G Myers |
| E-mail(s) |
tgm@nih.gov
|
| Organization name |
National Institute of Allergy and Infectious Diseases
|
| Department |
Research Technologies Branch
|
| Lab |
Genomic Technologies Section
|
| Street address |
50 South Drive, Room 5509
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-8005 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA224255 |
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