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| Status |
Public on Jun 29, 2006 |
| Title |
TGF-beta1 target genes in human hematopoietic stem/progenitor cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
CD34+ hematopoietic stem/progenitor cells were isolated from human cord blood and amplified in vitro for 10-14 days in serum-free medium with specific cytokines (Ju et al., Eur. J. Cell Biol. 82, 75-86, 2003; Hacker et al., Nat. Immunol. 4, 380-386, 2003). Cells were then treated with TGF-beta1 for various periods of time (2, 4, 16 hours) and RNA was prepared and subjected to microarray analysis.
Keywords: Response to cytokine treatment for various periods of time.
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| Overall design |
CD34+ hematopoietic stem/progenitor cells (HPC) were amplified in vitro and treated with TGF-beta1 (10 ng/ml) for 2, 4 and 16 hours.
HPC untreated
HPC + TGF-beta1 for 2 hours
HPC + TGF-beta1 for 4 hours
HPC + TGF-beta1 for 16 hours
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| Contributor(s) |
Ju X, Ruau D, Becker C, Zenke M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 26, 2006 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Martin Zenke |
| E-mail(s) |
Martin.Zenke@rwth-aachen.de
|
| Phone |
+49-241-80 80760
|
| Organization name |
Institute for Biomedical Engineering
|
| Department |
Cell Biology
|
| Street address |
Universitatsklinikum Aachen, RWTH
|
| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
|
| Samples (4)
|
| GSM116315 |
Hematopoietic stem/progenitor cells untreated |
| GSM116316 |
Hematopoietic stem/progenitor cells treated with TGF-beta1 for 2 hours |
| GSM116317 |
Hematopoietic stem/progenitor cells treated with TGF-beta1 for 4 hours |
| GSM116318 |
Hematopoietic stem/progenitor cells treated with TGF-beta1 for 16 hours |
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| This SubSeries is part of SuperSeries: |
| GSE5178 |
TGF-beta1 target genes in hematopoietic stem/progenitor cells and dendritic cells |
|
| Relations |
| BioProject |
PRJNA104281 |
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