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| Status |
Public on Dec 31, 2006 |
| Title |
Role of coactivator SRC-1/NcoA-1 for IL-6 target gene induction in HepG2 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This experiment series addresses the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. For that purpose, HepG2 human hepatocellular carcinoma cells were manipulated to stably express an shRNA that knocks down SRC-1 expression yielding the HepG2-∆Src1 cells. Either unmanipulated HepG2 or HepG2-∆Src1 cells were then treated for various periods with IL-6.
Keywords: time course, genetic modification
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| Overall design |
HepG2 or HepG2-∆Src1 (with silenced SRC-1/NcoA-1 expression) cells were either left untreated or treated with 10 ng/ml IL-6 for 1 or 4 hours. Every experiment was carried out in replicate.
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| |
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| Contributor(s) |
Horn F, Cvijic H |
| Citation(s) |
19203349 |
| Submission date |
May 22, 2006 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Friedemann Horn |
| E-mail(s) |
fhorn@medizin.uni-leipzig.de
|
| Organization name |
University of Leipzig
|
| Department |
Institute of Clinical Immunology and Transfusion Medicine
|
| Lab |
Molecular Immunology
|
| Street address |
Johannisalle 30
|
| City |
Leipzig |
| ZIP/Postal code |
04103 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA95779 |
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