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Series GSE4811 Query DataSets for GSE4811
Status Public on May 17, 2006
Title Comparison between classically activated and type II activated macrophages to identify novel markers
Organism Mus musculus
Experiment type Expression profiling by array
Summary The purpose of this study is to identify novel markers for the type II activated macrophage, which is generated by classical stimulation in the presence if IgG immune complexes. These cells gererally produce high levels of IL-10 and low levels of IL-12, in comparison to classically activated macrophages. We wish to identify gene expression which is enriched in Type II activated macrophages in comparison to classically activated macrophages.
Keywords: Stimulation comparison
 
Overall design The design of this experiment is to simulateously stimulate two popultions of macrophages and compare their gene expression. In this case, macrophages are primed overnight with IFN-gamma, washed, then stimulated with LPS (Classically) or LPS+Immune complexes (Type II).
 
Contributor(s) Edwards JP, Zhang X, Frauwirth KA, Mosser DM
Citation(s) 16905575
Submission date May 11, 2006
Last update date Feb 11, 2019
Contact name Justin Edwards
E-mail(s) jpe@umd.edu
Organization name University of Maryland
Department CBMG
Lab Mosser
Street address Blg. 231 Room 1105
City College Park
State/province MD
ZIP/Postal code 20742
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM108495 IFN gamma Macrophage_LPSstim_2h_rep1
GSM108496 macrophages_stimlpsic_2hours_rep1
GSM110213 IFN gamma Macrophage_LPSstim_2h_rep2
Relations
BioProject PRJNA95613

Significantly upregulated gene list header descriptions
Probe Set ID
Gene Symbol
Gene Title
Log Fold Change Array Set 1
Log Change Array Set 2
Fold Change Array Set 1
Fold Change Array Set 2
GO Biological Process Description
GO Molecular Function Description
GO Cellular Component Description

Data table
Probe Set ID Gene Symbol Gene Title Log Fold Change Array Set 1 Log Change Array Set 2 Fold Change Array Set 1 Fold Change Array Set 2 GO Biological Process Description GO Molecular Function Description GO Cellular Component Description
1452771_s_at Acsl3 acyl-CoA synthetase long-chain family member 3 1 1 2 2 lipid metabolism /// fatty acid metabolism /// metabolism magnesium ion binding /// catalytic activity /// long-chain-fatty-acid-CoA ligase activity /// ligase activity /// long-chain-fatty-acid-CoA ligase activity integral to membrane /// membrane
1450716_at Adamts1 a disintegrin-like and metallopeptidse (reprolysin type) with thrombospondin type 1 motif, 1 2.1 1.6 4.28709385 3.031433133 proteolysis /// integrin-mediated signaling pathway /// ovulation (sensu Mammalia) /// kidney development metalloendopeptidase activity /// heparin binding /// peptidase activity /// metallopeptidase activity /// zinc ion binding /// hydrolase activity /// metal ion binding /// protein binding /// metalloendopeptidase activity /// heparin binding extracellular matrix (sensu Metazoa) /// extracellular matrix /// basement membrane /// extracellular matrix /// cytoplasmic vesicle
1435181_at AI461788 expressed sequence AI461788 1.2 2.2 2.29739671 4.59479342 --- --- ---
1451341_s_at AI840826 expressed sequence AI840826 1.4 1 2.639015822 2 --- --- ---
1437072_at Arhgap25 Rho GTPase activating protein 25 1.1 1.2 2.143546925 2.29739671 --- GTPase activator activity ---
1454788_at Arl7 ADP-ribosylation factor-like 7 1.5 1.2 2.828427125 2.29739671 intracellular protein transport /// small GTPase mediated signal transduction /// protein transport /// rRNA processing /// ribosome biogenesis /// small GTPase mediated signal transduction nucleotide binding /// GTPase activity /// GTP binding nucleus /// intracellular /// nucleus
1436512_at Arl7 ADP-ribosylation factor-like 7 1.8 1.4 3.482202253 2.639015822 intracellular protein transport /// small GTPase mediated signal transduction /// protein transport /// rRNA processing /// ribosome biogenesis /// small GTPase mediated signal transduction nucleotide binding /// GTPase activity /// GTP binding nucleus /// intracellular /// nucleus
1424303_at AV216087 expressed sequence AV216087 2.7 1.7 6.498019171 3.249009585 intracellular signaling cascade DNA binding ---
1434409_at AW060994 expressed sequence AW060994 1.6 1.8 3.031433133 3.482202253 --- --- membrane /// integral to membrane
1440549_at B230334L07Rik RIKEN cDNA B230334L07 gene 2.1 1.5 4.28709385 2.828427125 --- --- ---
1452288_at BB128963 expressed sequence BB128963 2 1.1 4 2.143546925 phospholipid dephosphorylation inositol or phosphatidylinositol phosphatase activity ---
1424940_s_at BC022687 cDNA sequence BC022687 1.4 1.2 2.639015822 2.29739671 --- --- ---
1451533_at BC022687 cDNA sequence BC022687 1.6 2.1 3.031433133 4.28709385 --- --- ---
1415936_at Bcar3 breast cancer anti-estrogen resistance 3 1.6 1.6 3.031433133 3.031433133 intracellular signaling cascade /// small GTPase mediated signal transduction /// intracellular signaling cascade /// small GTPase mediated signal transduction guanyl-nucleotide exchange factor activity /// guanyl-nucleotide exchange factor activity intracellular
1418025_at Bhlhb2 basic helix-loop-helix domain containing, class B2 2.3 1.8 4.924577653 3.482202253 transcription /// regulation of transcription, DNA-dependent /// regulation of transcription /// negative regulation of transcription, DNA-dependent DNA binding /// protein binding /// protein binding /// transcriptional repressor activity /// transcription regulator activity /// DNA binding nucleus /// nucleus
1417045_at Bid BH3 interacting domain death agonist 1.8 1.4 3.482202253 2.639015822 apoptosis /// regulation of apoptosis /// positive regulation of apoptosis /// apoptosis /// regulation of apoptosis --- cytoplasm
1441534_at C86753 expressed sequence C86753 2.6 1.3 6.062866266 2.462288827 --- --- ---
1421228_at Ccl7 chemokine (C-C motif) ligand 7 1.1 1.8 2.143546925 3.482202253 transport /// chemotaxis /// inflammatory response /// immune response /// signal transduction /// sensory perception /// response to stimulus receptor activity /// cytokine activity /// transporter activity /// chemokine activity /// heparin binding extracellular region /// extracellular space /// membrane /// extracellular space
1416034_at Cd24a CD24a antigen 1.8 2.2 3.482202253 4.59479342 --- --- external side of plasma membrane /// membrane
1437502_x_at Cd24a CD24a antigen 2.8 2 6.964404506 4 --- --- external side of plasma membrane /// membrane

Total number of rows: 183

Table truncated, full table size 46 Kbytes.




Significantly downregulated gene list header descriptions
Probe Set ID
Gene Symbol
Gene Title
Log Fold Change Array Set 1
Log Fold Change Array Set 2
Fold Change Array Set 1
Fold Change Array Set 2
GO Biological Process Description
GO Molecular Function Description
GO Cellular Component Description

Data table
Probe Set ID Gene Symbol Gene Title Log Fold Change Array Set 1 Log Fold Change Array Set 2 Fold Change Array Set 1 Fold Change Array Set 2 GO Biological Process Description GO Molecular Function Description GO Cellular Component Description
1433454_at Abtb2 ankyrin repeat and BTB (POZ) domain containing 2 -2.3 -1.2 4.924577653 2.29739671 --- protein binding ---
1455161_at AI504432 expressed sequence AI504432 -1.6 -1.1 3.031433133 2.143546925 --- --- ---
1456064_at AI504432 expressed sequence AI504432 -1.5 -1.1 2.828427125 2.143546925 --- --- ---
1440790_x_at AL024069 Expressed sequence AL024069 (AL024069), mRNA -1.6 -1.2 3.031433133 2.29739671 --- --- ---
1444969_at AL024069 expressed sequence AL024069 -1.5 -1.2 2.828427125 2.29739671 --- --- ---
1439819_at AU015263 expressed sequence AU015263 -2 -1.4 4 2.639015822 --- --- ---
1454937_at B630005N14Rik RIKEN cDNA B630005N14 gene -1.3 -1.1 2.462288827 2.143546925 --- --- ---
1443526_at Bhc80 PHD finger protein 21A (Phf21a), mRNA -1.3 -1.1 2.462288827 2.143546925 transcription /// regulation of transcription, DNA-dependent /// regulation of transcription, DNA-dependent DNA binding /// metal ion binding /// protein binding /// zinc ion binding ---
1418854_at Birc2 baculoviral IAP repeat-containing 2 -1.6 -1.2 3.031433133 2.29739671 apoptosis /// anti-apoptosis /// protein ubiquitination /// regulation of apoptosis ubiquitin-protein ligase activity /// protein binding /// zinc ion binding /// metal ion binding /// protein binding ubiquitin ligase complex /// intracellular
1416122_at Ccnd2 cyclin D2 -2.3 -1.5 4.924577653 2.828427125 regulation of progression through cell cycle /// regulation of progression through cell cycle /// cell cycle /// cell division protein binding /// cyclin-dependent protein kinase regulator activity nucleus
1430127_a_at Ccnd2 cyclin D2 -2.3 -1.5 4.924577653 2.828427125 regulation of progression through cell cycle /// regulation of progression through cell cycle /// cell cycle /// cell division protein binding /// cyclin-dependent protein kinase regulator activity nucleus
1455956_x_at Ccnd2 cyclin D2 -2.2 -1.5 4.59479342 2.828427125 regulation of progression through cell cycle /// regulation of progression through cell cycle /// cell cycle /// cell division protein binding /// cyclin-dependent protein kinase regulator activity nucleus
1434745_at Ccnd2 Cyclin D2 (Ccnd2), mRNA -1.9 -1.3 3.732131966 2.462288827 regulation of progression through cell cycle /// regulation of progression through cell cycle /// cell cycle /// cell division protein binding /// cyclin-dependent protein kinase regulator activity nucleus
1448229_s_at Ccnd2 cyclin D2 -1.3 -1.1 2.462288827 2.143546925 regulation of progression through cell cycle /// regulation of progression through cell cycle /// cell cycle /// cell division protein binding /// cyclin-dependent protein kinase regulator activity nucleus
1446834_at Ctsc Cathepsin C (Ctsc), mRNA -1.9 -1.6 3.732131966 3.031433133 proteolysis cysteine-type endopeptidase activity /// dipeptidyl-peptidase I activity /// peptidase activity /// cysteine-type peptidase activity /// hydrolase activity extracellular space /// lysosome
1437939_s_at Ctsc cathepsin C -1.4 -1.1 2.639015822 2.143546925 proteolysis cysteine-type endopeptidase activity /// dipeptidyl-peptidase I activity /// peptidase activity /// cysteine-type peptidase activity /// hydrolase activity extracellular space /// lysosome
1443020_at F830020C16Rik RIKEN cDNA F830020C16 gene, mRNA (cDNA clone IMAGE:3490304) -1.1 -1.2 2.143546925 2.29739671 regulation of transcription, DNA-dependent /// transcription /// regulation of transcription, DNA-dependent /// regulation of transcription DNA binding /// transcription factor activity /// transcription factor activity /// sequence-specific DNA binding nucleus /// nucleus
1435477_s_at Fcgr2b Fc receptor, IgG, low affinity IIb -1.7 -1 3.249009585 2 negative regulation of type I hypersensitivity /// phagocytosis, engulfment /// defense response /// immune response /// cell surface receptor linked signal transduction /// humoral defense mechanism (sensu Vertebrata) /// negative regulation of B cell proliferation /// antigen presentation, exogenous antigen via MHC class II /// mast cell activation /// positive regulation of phagocytosis /// negative regulation of immune response receptor activity /// receptor signaling protein activity /// IgG binding /// IgG binding integral to plasma membrane /// external side of plasma membrane /// integral to membrane /// membrane /// integral to membrane
1442233_at Fyb FYN binding protein (Fyb), mRNA -1.1 -1.4 2.143546925 2.639015822 mast cell activation --- nucleus /// mitochondrion /// mitochondrial inner membrane
1417679_at Gfi1 growth factor independent 1 -3 -2.8 8 6.964404506 transcription /// regulation of transcription, DNA-dependent /// mechanosensory behavior /// inner ear morphogenesis /// auditory receptor cell differentiation /// negative regulation of cell fate specification /// positive regulation of cell fate specification /// cell fate commitment nucleic acid binding /// DNA binding /// zinc ion binding /// metal ion binding nucleus

Total number of rows: 49

Table truncated, full table size 14 Kbytes.




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