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| Status |
Public on Sep 05, 2014 |
| Title |
Identification of CITED2 target genes in human CB |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
CB CD34+ cells were were transduced with control (tNGFR) or CITED2 overexpression lentivectors, or control (shSCR) or shRNA CITED2 lentivectors and mRNA was isolated in order to investigate global gene expression changes upon perturbation of CITED2.
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| Overall design |
CB CD34+ cells were isolated by Miltenyi miniMACS column. Cells were prestimulated in HPGM with 100 ng/ml KITL, FLT3L and TPO for 12 hrs. Cells were transduced with control (tNGFR) or CITED2 overexpression lentivectors, or control (shSCR) shRNA CITED2 lentivectors, in three rounds over 48 hrs. Transduced cells were sorted after which RNA was isolated for Illumina beadhchip arrays HT12 v4.
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| Contributor(s) |
Schepers H |
| Citation(s) |
25184385 |
| Submission date |
May 23, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Hein Schepers |
| E-mail(s) |
h.schepers@umcg.nl
|
| Organization name |
University Medical Center Groningen
|
| Department |
Internal Medicine
|
| Lab |
Experimental Hematology
|
| Street address |
Hanzeplein 1
|
| City |
Groningen |
| State/province |
Groningen |
| ZIP/Postal code |
9713 GZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA205285 |
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