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| Status |
Public on May 31, 2013 |
| Title |
Candidate transcriptomic sources of inbreeding depression in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
The genomic causes of inbreeding depression are poorly known. Several studies have found widespread transcriptomic alterations in inbred organisms, but it remains unclear which of these alterations are causes of the depression and which are mere responses to the ensuing physiological stress.
We made a c-DNA microarray analysis in Drosophila melanogaster attempting to differentiate causes from responses of inbreeding depression. The rationale of the experiment was that, while depression is a general phenomenon producing similar consequences in different inbred lines, its first genetic causes would be different for each inbred line, as they are expected to be caused by the fixation of rare deleterious genes.
Many changes in expression were common to all sets, but fourteen genes, grouped in four expression clusters, showed strong set-specific changes, and were therefore candidates to be sources of the inbreeding depression observed.
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| Overall design |
We took four sets of inbred sublines, each set descending from a different founding pair obtained from a large outbred stock, and compared the expression of the three most depressed sublines and the three least depressed sublines from each set.
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| Contributor(s) |
García C, Avila V, Quesada H, Caballero A |
| Citation(s) |
23922905 |
| Submission date |
May 22, 2013 |
| Last update date |
Aug 13, 2019 |
| Contact name |
Humberto Quesada |
| E-mail(s) |
hquesada@uvigo.es
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| Organization name |
Universidad de Vigo
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| Department |
Bioquimica, Genetica e Inmunologia
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| Lab |
Genetics
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| Street address |
Facultad de Biologia. Campus Lagoas-Marcosende
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| City |
Vigo |
| State/province |
Galicia |
| ZIP/Postal code |
36310 |
| Country |
Spain |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (27)
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| GSM1146070 |
line5, subline -D, biological rep2 |
| GSM1146071 |
line5, subline -D, biological rep3 |
| GSM1146072 |
line7, subline +D, biological rep1 |
| GSM1146073 |
line7, subline +D, biological rep2 |
| GSM1146074 |
line7, subline +D, biological rep3 |
| GSM1146075 |
line7, subline -D, biological rep3 |
| GSM1146076 |
line12, subline +D, biological rep1 |
| GSM1146077 |
line12, subline +D, biological rep2 |
| GSM1146078 |
line12, subline +D, biological rep3 |
| GSM1146079 |
line12, subline -D, biological rep3 |
| GSM1146080 |
line15, subline +D, biological rep1 |
| GSM1146081 |
line15, subline +D, biological rep2 |
| GSM1146082 |
line15, subline +D, biological rep3 |
| GSM1146083 |
line15, subline -D, biological rep2 |
| GSM1146084 |
line15, subline -D, biological rep3 |
| GSM1146085 |
outbred control, biological rep1 |
| GSM1146086 |
outbred control, biological rep2 |
| GSM1146087 |
outbred control, biological rep3 |
| GSM1146088 |
line5, subline -D, biological rep1 |
| GSM1146089 |
line7, subline -D, biological rep2 |
| GSM1146090 |
line7, subline -D, biological rep1 |
| GSM1146091 |
line12, subline -D, biological rep2 |
| GSM1146092 |
line12, subline -D, biological rep1 |
| GSM1146093 |
line15, subline -D, biological rep1 |
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| Relations |
| BioProject |
PRJNA204981 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47176_RAW.tar |
54.4 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE47176_inbred_only_data.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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