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| Status |
Public on Jan 01, 2016 |
| Title |
miR-203, a microRNA of multilayered epithelia, inhibits metastatic fitness in human squamous cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The colonization of distant organs by metastatic carcinoma cells underpins most human cancer-related deaths, including those from head and neck squamous cell carcinoma (HNSCC). We report that miR-203, a miRNA that promotes keratinocyte differentiation, is necessary and sufficient to inhibit multiple post-extravasation events during HNSCC lung metastasis, including initial survival/engraftment, escape from metastatic dormancy, and overt colonization in vivo. Restoration of miR-203 expression in established lung metastases reduces overall metastatic burden. Instead of promoting differentiation, miR-203 controls lung metastasis through direct targeting of genes involved in cytoskeletal dynamics (LASP1), ECM remodeling (SPARC), and cell metabolism (NUAK1). Expression of miR-203 and its downstream targets correlates with HNSCC overall survival outcomes, suggesting the therapeutic potential of targeting this signaling axis.
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| Overall design |
Total RNA (including small RNAs) was isolated from cultured cells stably infected in biological duplicate with either a scrambled control hairpin or miR-203. Samples were harvested in technical duplicate.
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| Contributor(s) |
Benaich N, Menon S, Watt F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 16, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Nathan Benaich |
| Organization name |
King's College London
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| Department |
Centre for Stem Cells and Regenerative Medicine
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| Lab |
28th Floor, Tower Wing, Guy's Hospital
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| Street address |
Great Maze Pond
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| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (16)
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| GSM1143129 |
SCC13 control cells, 13_EV_4 |
| GSM1143130 |
SCC13 miR-203 overexpressing cells, 13_203_1 |
| GSM1143131 |
SCC13 miR-203 overexpressing cells, 13_203_2 |
| GSM1143132 |
SCC13 miR-203 overexpressing cells, 13_203_3 |
| GSM1143133 |
SCC13 miR-203 overexpressing cells, 13_203_4 |
| GSM1143134 |
SJG15 control cells, 15_EV_1 |
| GSM1143135 |
SJG15 control cells, 15_EV_2 |
| GSM1143136 |
SJG15 control cells, 15_EV_3 |
| GSM1143137 |
SJG15 control cells, 15_EV_4 |
| GSM1143138 |
SJG15 miR-203 overexpressing cells, 15_203_1 |
| GSM1143139 |
SJG15 miR-203 overexpressing cells, 15_203_2 |
| GSM1143140 |
SJG15 miR-203 overexpressing cells, 15_203_3 |
| GSM1143141 |
SJG15 miR-203 overexpressing cells, 15_203_4 |
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| Relations |
| BioProject |
PRJNA203379 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47028_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE47028_non-normalized.txt.gz |
4.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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