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| Status |
Public on Apr 30, 2013 |
| Title |
Ethanol effect on myoblast differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Expression profiling of C2C12 myoblast cells treated with ethanol during differentiation. Ethanol inhibits C2C12 differentiation. Results provide insight into signaling pathways altered by ethanol during differentiation.
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| Overall design |
When C2C12 cells reached 70% confluence in growth medium containing 20% FBS, culture medium was changed to differentiation medium containing 2% horse serum with and without 100mM ethanol. Samples were harvested from day zero (just prior to differentiation) as well as days 1, 2 and 3 following onset of differentiation with and without alcohol treatment. RNA was isolated using the Qiagen RNA mini-kit.
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| |
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| Contributor(s) |
Arya MA, Tai AK, Wooten EC, Parkin CD, Kudryavtseva E, Huggins GS |
| Citation(s) |
23977095 |
| Submission date |
Apr 29, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Michelle Arya |
| E-mail(s) |
michelle.arya8@gmail.com
|
| Organization name |
MCRI
|
| Street address |
15 Kneeland Street
|
| City |
Boston |
| ZIP/Postal code |
02111 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (24)
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| Relations |
| BioProject |
PRJNA200638 |
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