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| Status |
Public on Nov 01, 2014 |
| Title |
The Drosophila surface glia transcriptome: insights into conserved blood-brain barrier physiologies |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Maintenance of central nervous system (CNS) homeostasis requires tight regulation over the metabolites, drugs, cells, and pathogens entering the brain. The blood-brain barrier (BBB) carries out these functions, but the regulatory mechanisms underlying BBB physiology are not completely understood. In addition, the BBB has long been an obstacle to the pharmacologic treatment of CNS diseases, thus molecular model systems that can parse BBB functions and understand the complex integration of sophisticated cellular anatomy and highly polarized chemical protection physiology are desperately needed.
In this study, we developed FACS isolation methods for the purification of the surface glia that form the Drosophila BBB. By comparing the transcriptomes (via microarray analyses) of surface glia, FACS isolated neurons, and whole brains, we present a complete catalog of transcripts enriched at the Drosophila BBB. The surface glia transcriptome contains many ABC and SLC transporters, cell adhesion molecules, xenobiotic metabolism pathways, metabolic enzymes, and signaling molecules. Using gene set enrichment analyses and sequence-based comparisons, we compare the Drosophila surface glia to the vertebrate vascular endothelial BBB.
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| Overall design |
Each replicate for the microarray analysis represented sorted cells originating from different growth bottles and processed on different days. Five total replicates were run for each of the following genotypes: wildtype whole brain, and sorted GFP positive cells from Repo, Elav, and 9-137.
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| Contributor(s) |
DeSalvo MK, Bainton RJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 20, 2013 |
| Last update date |
May 04, 2018 |
| Contact name |
Michael DeSalvo |
| E-mail(s) |
desalvom@anesthesia.ucsf.edu
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| Organization name |
UC San Francisco
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| Department |
Department of Anesthesia
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| Lab |
Roland Bainton
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| Street address |
600 16 St Box 2200
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158-2200 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (20)
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| GSM1102719 |
Canton S, wildtype, whole brain, rep4 |
| GSM1102720 |
Canton S, wildtype, whole brain, rep5 |
| GSM1102721 |
FACS isolated CNS neurons, ELAV-GFP, rep1 |
| GSM1102722 |
FACS isolated CNS neurons, ELAV-GFP, rep2 |
| GSM1102723 |
FACS isolated CNS neurons, ELAV-GFP, rep3 |
| GSM1102724 |
FACS isolated CNS neurons, ELAV-GFP, rep4 |
| GSM1102725 |
FACS isolated CNS neurons, ELAV-GFP, rep5 |
| GSM1102726 |
FACS isolated CNS glia REPO-GFP, rep1 |
| GSM1102727 |
FACS isolated CNS glia REPO-GFP, rep2 |
| GSM1102728 |
FACS isolated CNS glia REPO-GFP, rep3 |
| GSM1102729 |
FACS isolated CNS glia REPO-GFP, rep4 |
| GSM1102730 |
FACS isolated CNS glia REPO-GFP, rep5 |
| GSM1102731 |
FACS isolated CNS surface glia 9.137-GFP, rep1 |
| GSM1102732 |
FACS isolated CNS surface glia 9.137-GFP, rep2 |
| GSM1102733 |
FACS isolated CNS surface glia 9.137-GFP, rep3 |
| GSM1102734 |
FACS isolated CNS surface glia 9.137-GFP, rep4 |
| GSM1102735 |
FACS isolated CNS surface glia 9.137-GFP, rep5 |
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| Relations |
| BioProject |
PRJNA193507 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45344_RAW.tar |
38.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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