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Status |
Public on Mar 19, 2013 |
Title |
Genome-wide analysis of gene expression in murine breast fibroblasts from normal tissue, hyperplasia, adenoma and carcinoma (MMTV-PyMT model) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Analysis of differences at gene expression level of hpvE6 immortalized fibroblasts isolated from normal mammary glands and from hyperplasia, adenoma and carcinoma stages using the MMTV-PyMT model (FVB/N background). Analysis demonstrated the activation of specific transcriptional programs in fibroblasts from later stages.
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Overall design |
Total RNA obtained from isolated hpvE6 immortalized fibroblasts from normal mammary gland (NF) and from different stages of tumour development using the MMTV-PyMT murine breast cancer model. Stages were hyperplasia (HpAF), adenoma (AdAF) and carcinoma (CAF). Fibroblasts were seeded in a deformable Matrigel:collagen I matrix and total RNA isolated 72h later.
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Contributor(s) |
Calvo F, Sahai E |
Citation(s) |
23708000 |
Submission date |
Mar 18, 2013 |
Last update date |
Jun 14, 2018 |
Contact name |
Fernando Calvo |
E-mail(s) |
fernando.calvo@cancer.org.uk
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Organization name |
Cancer Research UK
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Department |
London Research Institute
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Lab |
Tumour Cell Biology
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Street address |
44 Lincoln's Inn Fields
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City |
London |
ZIP/Postal code |
WC2A 3LY |
Country |
United Kingdom |
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Platforms (1) |
GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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Samples (16)
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Relations |
BioProject |
PRJNA193324 |