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| Status |
Public on Mar 29, 2006 |
| Title |
Expression Studies of Melanotransferrin in Mouse Brain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Melanotransferrin (MTf) or melanoma tumor antigen p97 is an iron (Fe)-binding transferrin homolog expressed highly on melanomas and at lower levels on normal tissues. It has been suggested that MTf is involved in a variety of processes such as Fe metabolism and cellular differentiation. Considering the crucial role of Fe in many metabolic pathways e.g., DNA synthesis, it is important to understand the function of MTf. To define the roles of MTf, a MTf knockout (MTf -/-) mouse model was developed. Examination of the MTf -/- mice demonstrated no phenotypic differences compared to wild-type littermates. However, microarray analysis showed differential expression of molecules involved in proliferation such as Mef2a, Tcf4, Gls and Apod in MTf -/- mice compared to MTf +/+ littermates, suggesting a role for MTf in proliferation and tumorigenesis.
Keywords: Melanotransferrin, knockout mouse, comparative genomic hybridization
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| Overall design |
Six 17.5 week old female mice comprising four MTf -/- and two MTf +/+ from the same litter were used. Total RNA was isolated from brain tissue and processed as per standard Affymetrix protocol to obtain final cRNA products that were hybridized to the mouse GeneChip® 430 2.0. The mouse GeneChip® 430 2.0 consists of greater than 39,000 transcripts and variants from over 34,000 well characterized mouse genes. On completion of hybridization and washing, microarray chips were scanned with the Affymetrix GeneChip® Scanner 3000 (Millenium Sciences).
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| Contributor(s) |
Suryo Rahmanto Y, Dunn LL, Sekyere EO, Richardson DR |
| Citation(s) |
16704991, 17449903 |
| Submission date |
Mar 23, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Des Raymond Richardson |
| E-mail(s) |
d.richardson@med.usyd.edu.au
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| Organization name |
University of Sydney
|
| Department |
Bosch Institute
|
| Lab |
Pathology
|
| Street address |
92-94 Parramatta Rd, Sydney
|
| City |
Sydney |
| State/province |
New South Wales |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM101412 |
Brain_Melanotransferrin Knockout_affs275-1096 |
| GSM101413 |
Brain_Melanotransferrin Knockout_affs275-1097 |
| GSM101414 |
Brain_Melanotransferrin Knockout_affs275-1098 |
| GSM101415 |
Brain_Melanotransferrin Knockout_affs275-1101 |
| GSM101416 |
Brain_C57 Wildtype_affs275-1099 |
| GSM101417 |
Brain_C57 Wildtype_affs275-1100 |
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| Relations |
| BioProject |
PRJNA94611 |
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