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| Status |
Public on Feb 08, 2013 |
| Title |
Posterior Second Heart Field (pSHF) Transcriptomes of Wild-type and Tbx5 Mutant Mouse Embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To understand Tbx5-Hedgehog molecular networks in the posterior second heart field (pSHF), we have employed whole genome microarray expression profiling as a discovery platform to identify genes with Tbx5-dependent expression changes. We microdissected the pSHF from E9.5 embryos and compared the Tbx5 mutant samples with than of wild-type using Agilent 4x44k Mouse Whole Genome Arrays (n = 4 WT pools and 4 Tbx5 -/+ pools).
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| Overall design |
Microdissected pSHF from E9.5 mouse embryos was molecularly verified by real-time PCR. Tbx5 mutant embryos were compared with wild-type embryos. Four independnet pools of RNAs from each biological group were measured on 1-color Agilent Mouse Whole Genome Arrays (n = 4 WT pools and 4 Tbx5 -/+ pools).
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| Contributor(s) |
Yang X, Hoffmann AD, Moskowitz IP |
| Citation(s) |
25356765 |
| Submission date |
Jan 17, 2013 |
| Last update date |
Jan 02, 2019 |
| Contact name |
Xinan "Holly" Yang |
| E-mail(s) |
xyang2@uchicago.edu
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| Phone |
773-702-5960
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| Organization name |
universty of Chicago
|
| Street address |
KCBD5121, 900 E. 57th Str.
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE44756 |
Hedgehog Molecular Networks in the Second Heart Field |
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| Relations |
| BioProject |
PRJNA186796 |
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