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| Status |
Public on Mar 07, 2013 |
| Title |
in-vitro differentiated midbrain dopaminergic neurons from isogenic human induced pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We have generated isogenic induced pluripotent stem cell lines by reprogramming human fibroblasts from patients carrying the LRRK2 G2019S mutation with subsequent zinc finger nuclease - mediated targeted correction of the diseased allele.
These iPS cell lines were differentiated for 30 days using a direct differentiation protocol towards midbrain dopaminergic neurons (mDANs).
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| |
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| Overall design |
Isogenic human iPS cells carrying the LRRK2 WT and G2019S locus were differentiated to dopaminergic neurons to detect gene expression changes associated with mutated LRRK2.
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| Citation(s) |
23472874 |
| Submission date |
Jan 08, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Peter Reinhardt |
| E-mail(s) |
peter.reinhardt@mpi-muenster.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Street address |
Von-Esmarch-Straße 54
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA185600 |
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