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| Status |
Public on Nov 01, 2013 |
| Title |
The Cytokines Interleukin-1 and Interferon-gamma Differentially Program Beta-glucan–activated Dendritic Cells via IkappaB-zeta Modulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To evaluate the DC genome-wide gene expression in response to beta-glucan and its regulation by IL-1 receptor antagonist (IL-1RA) we used a whole genome microarray. The gene expression profiling was performed in DC left untreated or exposed to beta-glucan for 4 and 12 h, in absence or presence of IL-1RA. This strategy allowed the identification of early/immediate and late/secondary genes regulated by beta-glucan in an IL-1-dependent and -independent manner. Human monocyte-derived DC were obtained by a 6/7-d cultures of freshly isolated monocytes with recombinant human IL-4 (10 ng/ml) and GM-CSF (50 ng/ml).
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| Overall design |
Beta-glucan-associated gene expression and its regulation by IL-1RA in human DC was measured in cells left untreated or at 4 and 12 h after exposure to 10 ug/ml of particulate beta-glucan in absence or presence of 2.5 ug/ml of IL-1RA. Five different conditions (Untreated 0h, beta-glucan 4h, IL-1RA + beta-glucan 4h, beta-glucan 12h, and IL-1RA + beta-glucan 12h) were tested using DC from three different donors.
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| Contributor(s) |
Trinchieri G, Cardone M |
| Citation(s) |
25474109 |
| Submission date |
Nov 09, 2012 |
| Last update date |
Oct 17, 2019 |
| Contact name |
Marco Cardone |
| E-mail(s) |
cardonem@mail.nih.gov
|
| Phone |
3012284387
|
| Organization name |
NCI/NIH
|
| Department |
CIP
|
| Street address |
1050 Boyles Street
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA179266 |
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