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| Status |
Public on Oct 16, 2012 |
| Title |
mRNA expression after siRNA-mediated knock down of Enhancer of zeste homolog 2 (Ezh2) in human umbilical vein endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
mRNA expression after Ezh2 knock down was analyzed to identify genes regulated by Ezh2.
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| Overall design |
Human umbilical vein endothelial cells (HUVEC) were transfected with 25 nmol/L of control small interfering RNA (siRNA) (Silencer Select Negative Control Ambion, Austin, TX) or siRNA directed against Ezh2 (s4918; Ambion) using Oligofectamine (Invitrogen). Total RNA was harvested 72 hours after transfection.
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| |
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| Contributor(s) |
Dreger H, Ludwig A, Weller A, Stangl V, Baumann G, Meiners S, Stangl K |
| Citation(s) |
22966008 |
| Submission date |
Oct 16, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Henryk Dreger |
| E-mail(s) |
henryk.dreger@charite.de
|
| Phone |
+49 30 450 613 496
|
| Fax |
+49 30 450 513 932
|
| Organization name |
Charité - Universitätsmedizin Berlin
|
| Department |
Cardiology and Angiology
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
D-10117 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (3) |
| GSM1020088 |
HUVEC_Ezh2- vs. control-siRNA_replicate 1 |
| GSM1020089 |
HUVEC_Ezh2- vs. control-siRNA_replicate 2 |
| GSM1020090 |
HUVEC_Ezh2- vs. control-siRNA_replicate 3 |
|
| Relations |
| BioProject |
PRJNA177684 |
