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| Status |
Public on Jan 01, 2013 |
| Title |
Gene expression profiling of Nodal versus Activin-derived endoderm from mouse ES cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To explore the molecular basis of functional differences observed between Nodal versus Activin-derived endoderm, we compared their respective gene expression profiles. Sox17-GFP mouse ES cells were differentiated in the presence of Nodal or Activin for 7 days, after which GFP(+) cells were purified by FACs. Undifferentiated ES cells were also included for comparison as a control. Results indicate that the two endoderm populations are nearly identical at the level of global transcription. Subtle differences suggest a difference in the degree of endoderm progression.
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| Overall design |
Total RNA obtained from SOX17-GFP (+) mouse ES cells, Sox17-GFP treated with Nodal, Sox17-GFP treated with Activin.
Two replicates for each sample; Sample data table contains average values of two replicates. The non_normalized.txt file contains individual signal values for each replicate.
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| Contributor(s) |
Chen AE, Melton DA |
| Citation(s) |
23293299 |
| Submission date |
Sep 22, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Alice E Chen |
| Organization name |
Harvard University
|
| Department |
Stem Cell and Regenerative Biology
|
| Lab |
Douglas Melton
|
| Street address |
7 Divinity Avenue
|
| City |
Cambridge |
| State/province |
Ma |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA175712 |
