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| Status |
Public on Sep 20, 2012 |
| Title |
Molecular profiling of activated neurons by phosphorylated ribosome capture [Illumina BeadArray] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, enriching for mRNAs that were selectively expressed in activated neurons
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| Overall design |
Mice were given an injection of concentrated salt solution (2M NaCl, 325 uL) or PBS, and then sacrificed 2h later and hypothalami dissected. Tissue homogenates were prepared from hypothalamus and phosphorylated ribosomes immunoprecipitated using pS6 244/247 antibodies. The immunoprecipitated RNA and total RNA from both control and salt treated animals were then purified and analyzed by Illumina Microarray.
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| Contributor(s) |
Knight ZA, Friedman JM |
| Citation(s) |
23178128 |
| Submission date |
Sep 19, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Zachary Knight |
| Organization name |
Rockefeller University
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE40995 |
Molecular profiling of activated neurons by phosphorylated ribosome capture |
|
| Relations |
| BioProject |
PRJNA175511 |
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