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| Status |
Public on Sep 13, 2012 |
| Title |
Comparison of gene expression profilling between iPS vs SSEA-1 positive or SSEA-1/3 negative sorted N417 cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To prove that hypoxia generates Cancer stem cells (CSCs) from non-CSCs, we needed to separate out already existing tumorigenic cells from the cell population. To identify CSCs, we used ES cell-specific cell surface antigens (SSEA-1, -3, -4, and Tra1-81) on the assumption that the immature cell fraction would be rich in CSCs. We analyzed the expression levels of such embryonic antigens and stemness genes in cells exposed to hypoxia.
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| |
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| Overall design |
iPS (253G1), SSEA-1 positive-N417 3 of SSEA-1/3 double negative-N417, each condition is represented by 3 biological replicates
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| Contributor(s) |
Suzuki M, Iida H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 12, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Mitsuhiro Suzuki |
| E-mail(s) |
misuzuki@med.uoeh-u.ac.jp
|
| Phone |
+81-93-691-7236
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| Organization name |
University of Occupational and Environmental Health, Japan
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| Department |
Biochemistry
|
| Street address |
1-1 Iseigaoka, Yahatanisi-ku
|
| City |
Kitakyushu |
| State/province |
Fukuoka |
| ZIP/Postal code |
807-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA175065 |
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