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| Status |
Public on Aug 28, 2012 |
| Title |
The downstream molecules of Notch signaling in vascular endothelial cell senescence |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To further investigate the mechanism how the decline in Notch signaling induces premature senescence in endothelial cells, we performed microarray analysis and identified Id1 nd DUSP1 as the downstream molecules of Notch pathway. In quantitative PCR and western blot analyses, the expression level of Id1 and DUSP1 increased in Notch1 over-expressing endothelial cells and decreased in knockdown similar to the result of microarray.
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| Overall design |
The gene expression of human unbilical endothelial vein cells (HUVEC) infected with retroviral vectors encoding Jagged1, Jagged1-shRNA, or Notch1-shRNA. HUVEC infected with empty vector was used as a control. In each genotypes, three independent lines at passage 8 were performed.
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| Contributor(s) |
Yamashita M, Yoshida Y, Sato K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 27, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Yohko Yoshida |
| E-mail(s) |
yohko105@yahoo.co.jp
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| Organization name |
Chiba University Graduate school of medicine
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| Street address |
1-8-1, Inohana, Chuo-ku
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| City |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA173881 |
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