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| Status |
Public on Oct 06, 2013 |
| Title |
Gene expression in an EMT model using an immortalized bronchial epithelial cell line induced by TGF-β1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This study aimed to establish an epithelial-mesenchymal transition (EMT) model with an immortalized human bronchial epithelial cell line, M-BE, and to identify an EMT signature gene set. The TGF-β1-induced M-BE cells got spindle-shaped fibroblast-like morphology and lost the cell-cell contact, with down-regulated expression of epithelial marker E-cadherin and up-regulated expression of mesenchymal markers N-cadherin and Vimentin. Examined by microarray, there were 2628 genes identified as significant EMT-related, including 1490 up-regulated genes (FC > 2, fdr < 0.01) and 1138 down-regulated genes (FC < 0.5, fdr < 0.01) in TGF-β1-induced M-BE cells.
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| Overall design |
M-BE cells were treated with human recombinant TGF-β1 (5ng/ml) for six days. M-BE cells cultured without TGF-β1 were considered the controls. Three replicates of each were carried out for this investigation. Agilent 4x44K Human Whole Genome expression microarray (G4112F) analysis was applied to the RNA samples of the TGF-β1-treated M-BE cells and the controls.
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| Contributor(s) |
Cao B, Feng L, Lu D, Guo S, Cheng S, Gao Y, Zhang K |
| Citation(s) |
28881838 |
| Submission date |
Aug 24, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Bangrong Cao |
| E-mail(s) |
caobangronghe@163.com
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| Organization name |
Cancer Institute & Hospital, Chinese Academy of Medical Sciences
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| Street address |
NO.17 Panjiayuannanli, Chaoyang District, Beijing, P.R.China
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| City |
Beijing |
| ZIP/Postal code |
100021 |
| Country |
China |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA173793 |
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