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| Status |
Public on Apr 25, 2013 |
| Title |
HUVECs with endogenous or overexpressed GPR4 treated with pH 8.4 (control) or pH 6.4 for 5 h |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Acidic tissue microenvironment is commonly found in a variety of pathophysiological conditions. GPR4 is a proton-sensing G protein-coupled receptor that is fully activated by acidic extracellular pH but has lesser activity at the physiological pH 7.4 and minimal activity at more alkaline pH.
To determine the effects of GPR4 activation by acidosis on vascular endothelial cells, we examined the global gene expression of the acidosis response in primary human umbilical vein endothelial cells (HUVEC) with varying level of GPR4.
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| Overall design |
HUVEC with endogenous or overexpressed GPR4 level (designated as HUVEC/Vector & HUVEC/GPR4 cells). Two treatment conditions: pH 6.4 vs. pH 8.4 for 5 h. Biological replicates: 4 HUVEC/Vector replicates (pH6.4 vs pH 8.4), and 4 HUVEC/GPR4 replicates (pH6.4 vs pH 8.4).
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| Contributor(s) |
Dong L, Yang LV |
| Citation(s) |
23613998 |
| Submission date |
Aug 12, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Lixue Dong |
| E-mail(s) |
dongl@ecu.edu
|
| Organization name |
East Carolina University
|
| Street address |
600 Moye Blvd.
|
| City |
Greenville |
| State/province |
NC |
| ZIP/Postal code |
27834 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (8)
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| GSM984510 |
HUVEC/Vector_pH 6.4-treated_5 h_rep1 |
| GSM984511 |
HUVEC/Vector_pH 6.4-treated_5 h_rep2 |
| GSM984512 |
HUVEC/Vector_pH 6.4-treated_5 h_rep3 |
| GSM984513 |
HUVEC/Vector_pH 6.4-treated_5 h_rep4 |
| GSM984514 |
HUVEC/GPR4_pH 6.4-treated_5 h_rep1 |
| GSM984515 |
HUVEC/GPR4_pH 6.4-treated_5 h_rep2 |
| GSM984516 |
HUVEC/GPR4_pH 6.4-treated_5 h_rep3 |
| GSM984517 |
HUVEC/GPR4_pH 6.4-treated_5 h_rep4 |
|
| Relations |
| BioProject |
PRJNA172639 |
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