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| Status |
Public on Sep 01, 2012 |
| Title |
Gene expression in primary isolated human myoblast during proliferation, G0 arrest, reactivation and differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A microarray study including two primary isolated human myoblast cell cultures ( samples A and B) was designed in order to detect the differences in global gene expression during four different culture conditions, two actively proliferating and two non-proliferating. Myoblasts were kept long term proliferating (BG0, before G0) followed by G0 arrest (G0), reactivation (AG0, after G0) and finally they were differentiated (Dif). The samples BG0 and AG0 were harvested when cells reached 70-80% confluence. The raw data from gene arrays were normalized, sample A and B were paired and the mean expression value used for further analyses. The four samples were compared to each other in order to detect differences in gene expression between the samples.
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| Overall design |
Primary isolated human myoblast cells were cultured through four different culture, BG0, G0, AG0 and Dif. Two independent studies were made with primary isolated myoblasts from two different individuals for each of the four culture condition. Thus the number of samples for microarray analyses was 8.
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| Contributor(s) |
Sellathurai J, Cheedipudi S, Dhawan J, Schroeder HD |
| Citation(s) |
23717533 |
| Submission date |
Jun 18, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Genotypic technology |
| E-mail(s) |
sudha.rao@genotypic.co.in
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| Organization name |
Genotypic Technology
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| Street address |
259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE
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| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560094 |
| Country |
India |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA168644 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38769_RAW.tar |
71.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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