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| Status |
Public on Jun 01, 2013 |
| Title |
IL-27, PXN, CXCR4, GZMA, PRF1 and Foxp3 genes are differentially expressed in CD4+ T cells of HTLV-1-infected individuals. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
HTLV-1 preferentially infects CD4+ T cells and these cells play a central role in HTLV-1 infection. In this study, we investigated the global gene expression profile of circulating CD4+ T cells from distinct clinical status of HTLV-1-infected individuals in regard to Tax expression levels. CD4+ T cells were isolated from asymptomatic HTLV-1 carrier (HAC) and HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients, in order to identify genes involved in the HAM/TSP development. Hierarchical clustering analysis showed that healthy controls (CT) and HTLV-1-infected samples clustered separately. We also observed that HAC and HAM/TSP groups clustered separately regardless Tax expression. The gene expression profile of CD4+ T cells was compared among CT, HAC and HAM/TSP groups. The IL-27, PXN, CXCR4, GZMA, PRF1 and Foxp3 genes were differentially expressed between HAC and HAM/TSP groups and the frequency of CD4+Foxp3+ regulatory T cells (Treg) were higher in HTLV-1-infected individuals. These findings suggest that CD4+ T cells activity is distinct between HAC and HAM/TSP groups as expected.
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| Overall design |
In order to study the transcriptional changes in CD4 T cell from HTLV-1-infected individuals, immunomagnetically purified CD4+ T-cells from the peripheral blood of 4 asymptomatic HTLV-1 carrier individuals (HAC) and 4 individuals with HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP), as well as from 4 healthy controls (CT) were isolated and processed the microarray assay according Agilent's protocol. The differential expressed genes, molecular characterization and networks analysis were evaluated using robust bioinformatic tools, then the real time PCR was done to validate the genes.
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| Contributor(s) |
Pinto MT, Malta TM, Rodrigues ES, Pinheiro DG, Panepucci RA, Malmegrim de Farias KC, Sousa AP, Takayanagui OM, Tanaka Y, Covas DT, Kashima S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 06, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Daniel Guariz Pinheiro |
| E-mail(s) |
dgpinheiro@usp.br
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| Phone |
+55-16-2101-9300
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| Organization name |
Faculdade de Medicina de Ribeirão Preto (Universidade de São Paulo)
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| Department |
Departamento de Genética
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| Lab |
Laboratório de Genética Molecular e Bioinformática
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| Street address |
Rua Tenente Catão Roxo, 2501
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| City |
Ribeirão Preto |
| State/province |
São Paulo |
| ZIP/Postal code |
14051-140 |
| Country |
Brazil |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA168100 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38537_RAW.tar |
105.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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