|
| Status |
Public on May 01, 2006 |
| Title |
Complete embryonic E11.5 metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
E11.5 metanephric mesenchyme and ureteric bud were dissected from the E11.5 kidney rudiment using fine manual microdissection (ureteric bud only) or both fine manual microdissection and laser capture microdissection (metanephric mesenchyme) to define the gene expression profiles of these structures. Additionally, HoxA11, HoxD11 compound null E11.5 metanephric mesenchyme was obtained through laser capture microdissection allowing analysis of possible Hox targets in kidney development. Targets from multiple biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430_v2 arrays.
Keywords: embryonic metanephric kidney, kidney development, Hoxa11, Hoxd11, compound null targeted mice
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| Overall design |
Using microdissection techniques, ureteric bud and metanephric mesenchyme were dissected from E11.5 kidney rudiments allowing the identificated genes specifically regulated in either structure. In addition, Hoxa11, Hoxd11 compound null E11.5 metanephric mesenchyme were normalized to wild type embryonic controls allowing the identification of potential Hox targets in normal kidney development. Each structure/genotype were represented in biological (seperate embryo) replicate.
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| |
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| Contributor(s) |
Schwab K, Potter SS |
| Citation(s) |
16581055 |
| Submission date |
Dec 14, 2005 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Kristopher Robert Schwab |
| E-mail(s) |
kristopher.schwab@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital Research Foundation
|
| Department |
Developmental Biology
|
| Lab |
S. Steven Potter
|
| Street address |
3333 Burnett Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (16)
|
| GSM87677 |
HoxA11, D11 double null embryonic E11.5 Metanephric Mesenchyme replicate 1 |
| GSM87678 |
Wild type embryonic E11.5 Metanephric Mesenchyme replicate 5 |
| GSM87679 |
Wild type embryonic E11.5 Ureteric Bud replicate 1 |
| GSM87680 |
Wild type embryonic E11.5 Ureteric Bud replicate 2 |
| GSM87681 |
Wildtype embryonic E11.5 Metanephric Mesenchyme replicate 1 |
| GSM87682 |
Wild type embryonic E11.5 Metanephric Mesenchyme replicate 6 |
| GSM87683 |
Wildtype embryonic E11.5 Metanephric Mesenchyme replicate 2 |
| GSM87684 |
Wild type embryonic E11.5 Ureteric Bud replicate 3 |
| GSM87685 |
Wild type embryonic E11.5 Ureteric Bud replicate 4 |
| GSM87686 |
Wildtype embryonic E11.5 Metanephric Mesenchyme replicate 3 |
| GSM87687 |
Wild type embryonic E11.5 Metanephric Mesenchyme replicate 7 |
| GSM87688 |
Wildtype embryonic E11.5 Metanephric Mesenchyme replicate 4 |
| GSM87689 |
HoxA11, D11 double null embryonic E11.5 Metanephric Mesenchyme replicate 2 |
| GSM87690 |
HoxA11, D11 double null embryonic E11.5 Metanephric Mesenchyme replicate 3 |
| GSM87691 |
HoxA11, D11 double null embryonic E11.5 Metanephric Mesenchyme replicate 4 |
| GSM87692 |
HoxA11, D11 double null embryonic E11.5 Metanephric Mesenchyme replicate 5 |
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| Relations |
| BioProject |
PRJNA94039 |
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