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| Status |
Public on Feb 04, 2023 |
| Title |
NPC stem-like cells [Agilent] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Stem-like cells of nasopharyngeal cancer were enriched by 3 different methods: 1) Side population (SP) assay, 2) LMP2A over-expression and 3) spheroid culture. SP cells and LMP2A over-expressed cells were inoculated into immunocompromised mice for xenograft tumors. Transcriptomes of all 5 of these in vitro and in vivo generated NPC stem-like cells were then analyzed and compared to their counterparts by microarray. All samples were analyzed in the Agilent platform except the SP cells and their counterpart (analyzed in the Affymetrix platform).
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| Overall design |
Two to three RNA sample replicates were prepared from each type of the NPC stem-like cells and their counterpart. Dye swap was performed to avoid bias. Each Agilent array was loaded with equal amount of 2 differentially labeled c-RNAs for hybridization. After conversion of scanned microarray images into signal intensities and dye differences corrected in Feature extraction, all analysis was performed in the single color analysis module in GeneSpring.
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| Contributor(s) |
Tan LP, Hoe SL, Fazlyn R, Ahmad SK, Chu TL, Nurul AS, Munirah A, Khoo AB |
| Citation missing |
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| Submission date |
Feb 28, 2012 |
| Last update date |
Feb 04, 2023 |
| Contact name |
Lu Ping Tan |
| E-mail(s) |
luping@imr.gov.my
|
| Organization name |
Institute for Medical Research
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| Department |
Molecular Pathology Unit
|
| Street address |
Jalan Pahang
|
| City |
Kuala Lumpur |
| ZIP/Postal code |
50588 |
| Country |
Malaysia |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA155787 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36124_US45103080_251485038674_S01_GE2_107_Sep09_1_1.txt.gz |
4.2 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485038674_S01_GE2_107_Sep09_1_2.txt.gz |
4.3 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485038674_S01_GE2_107_Sep09_1_3.txt.gz |
4.2 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485038674_S01_GE2_107_Sep09_1_4.txt.gz |
4.2 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485038675_S01_GE2_107_Sep09_1_1.txt.gz |
4.2 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485038675_S01_GE2_107_Sep09_1_2.txt.gz |
4.3 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485038675_S01_GE2_107_Sep09_1_3.txt.gz |
4.2 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485038675_S01_GE2_107_Sep09_1_4.txt.gz |
4.2 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485045478_S01_GE2_107_Sep09_1_4.txt.gz |
4.3 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485045479_S02_GE2-v5_95_Feb07_1_1.txt.gz |
15.4 Mb |
(ftp)(http) |
TXT |
| GSE36124_US45103080_251485045479_S02_GE2-v5_95_Feb07_1_2.txt.gz |
15.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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