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Series GSE35287 Query DataSets for GSE35287
Status Public on Jan 24, 2012
Title Unique Transcriptome, Pathways, and Networks in the Human Endometrial Fibroblast Response to Progesterone in Endometriosis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Eutopic endometrium in endometriosis has molecular evidence of resistance to progesterone (P4) and activation of the PKA pathway in the stromal compartment. To investigate global and temporal responses of eutopic endometrium to P4, we compared early (6-h), intermediate (48-h), and late (14-day) transcriptomes, signaling pathways, and networks of human endometrial stromal fibroblasts (hESFs) from women with endometriosis (hESFendo) to hESFs from women without endometriosis (hESFnonendo). Endometrial biopsy samples were obtained from subjects with and without mild peritoneal endometriosis (n = 4 per group), and hESFs were isolated and treated with P4 (1 μM) plus estradiol (E2) (10 nM), E2 alone (10 nM), or vehicle for up to 14 days. Total RNA was subjected to microarray analysis using a Gene 1.0 ST (Affymetrix) platform and analyzed by using bioinformatic algorithms, and data were validated by quantitative real-time PCR and ELISA. Results revealed unique kinetic expression of specific genes and unique pathways, distinct biological and molecular processes, and signaling pathways and networks during the early, intermediate, and late responses to P4 in both hESFnonendo and hESFendo, although a blunted response to P4 was observed in the latter. The normal response of hESF to P4 involves a tightly regulated kinetic cascade involving key components in the P4 receptor and MAPK signaling pathways that results in inhibition of E2-mediated proliferation and eventual differentiation to the decidual phenotype, but this was not established in the hESFendo early response to P4. The abnormal response of this cell type to P4 may contribute to compromised embryonic implantation and infertility in women with endometriosis.
 
Overall design We compared early (6-h), intermediate (48-h), and late (14-day) in vitro whole-genome responses of hESF from women with endometriosis (hESFendo) to hESF from women without endometriosis (hESFnonendo) treated with P4 plus E2 (E2P4), E2 alone, or vehicle alone. Using this experimental paradigm, the data demonstrate unique phenotypes, gene expression processes, biochemical and signaling pathways, and networks suggestive of early, intermediate, and late responses of hESFnonendo and hESFendo to P4, giving insights into the complexity of events occurring normally in response to P4 and in the setting of endometriosis.
 
Contributor(s) Aghajanova L, Tatsumi K, Horcajadas JA, Zamah AM, Esteban FJ, Herndon CN, Conti M, Giudice LC
Citation(s) 20864642
Submission date Jan 23, 2012
Last update date Jul 26, 2018
Contact name Linda Giudice
E-mail(s) giudice@obgyn.ucsf.edu
Phone 415-476-2039
Organization name University of California, San Francisco
Department OBGYN and RS
Lab Giudice Lab
Street address 513 Parnassus Ave. HSE 1619
City San Francisco
State/province CA
ZIP/Postal code 94122
Country USA
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (80)
GSM865132 Endometrial tissue-isolated human endometrial stromal fibroblast (hESF) 21
GSM865133 Endometrial tissue-isolated human endometrial stromal fibroblast (hESF) 22
GSM865134 Endometrial tissue-isolated human endometrial stromal fibroblast (hESF) 23
Relations
BioProject PRJNA151587

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35287_RAW.tar 370.2 Mb (http)(custom) TAR (of CEL)
GSE35287_final_normalized_data.txt.gz 9.8 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
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