|
| Status |
Public on Jan 16, 2012 |
| Title |
Primary TNBC tumor in the presence or absence of SHP2 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The first bona fide PTP proto-oncogene was the Src-homology 2 domain-containing phosphatase SHP2 (encoded by PTPN11), an ubiquitously expressed PTP that transduces mitogenic, pro-survival, cell fate and/or pro-migratory signals from numerous growth factor-, cytokine- and extracellular matrix receptors. In malignancies, SHP2 is hyperactivated either downstream of oncoproteins or by mutations.We provide analysis of a primary triple-negative breast tumor grown as xenografts in the presence or absence of SHP2 for 30 days.
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| Overall design |
A primary triple-negative breast tumor (BT8) was transduced with a doxycycline-inducible lentiviral vector expressing a CTRL miR or SHP2 miR1 or SHP2 miR2. Cells from each group were injected in imuunodeficient mice and after tumor development, the knockdown of SHP2 was induced for 30 days in vivo. At day 30, tumors were dissected and RNA isolated for gene expression analysis.
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| |
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| Contributor(s) |
Aceto N, Thiry S, Bentires-Alj M |
| Citation(s) |
22388088 |
| Submission date |
Jan 15, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Tim Roloff |
| E-mail(s) |
tim.roloff@fmi.ch
|
| Organization name |
FMI
|
| Department |
Functional Genomics
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (14)
|
| GSM862274 |
Primary TNBC (BT8) xenograft_Parental_1 |
| GSM862275 |
Primary TNBC (BT8) xenograft_Parental_2 |
| GSM862276 |
Primary TNBC (BT8) xenograft_CTRL miR_1 |
| GSM862277 |
Primary TNBC (BT8) xenograft_CTRL miR_2 |
| GSM862278 |
Primary TNBC (BT8) xenograft_CTRL miR_3 |
| GSM862279 |
Primary TNBC (BT8) xenograft_CTRL miR_4 |
| GSM862280 |
Primary TNBC (BT8) xenograft_SHP2 miR1_1 |
| GSM862281 |
Primary TNBC (BT8) xenograft_SHP2 miR1_2 |
| GSM862282 |
Primary TNBC (BT8) xenograft_SHP2 miR1_3 |
| GSM862283 |
Primary TNBC (BT8) xenograft_SHP2 miR1_4 |
| GSM862284 |
Primary TNBC (BT8) xenograft_SHP2 miR2_1 |
| GSM862285 |
Primary TNBC (BT8) xenograft_SHP2 miR2_2 |
| GSM862286 |
Primary TNBC (BT8) xenograft_SHP2 miR2_3 |
| GSM862287 |
Primary TNBC (BT8) xenograft_SHP2 miR2_4 |
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| This SubSeries is part of SuperSeries: |
| GSE34525 |
BT474 tumors, primary TN tumors and MCF10A-HER2/3 cells in the presence or absence of SHP2 |
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| Relations |
| BioProject |
PRJNA156015 |
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