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| Status |
Public on Aug 23, 2012 |
| Title |
Global gene expression analysis of human embryonic stem cells, adult fibroblasts , and CD34+ cord blood (CB) cells before, during, and afer their episomal induction of pluripotency |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Global gene expression analysis of (a) human embryonic stem cells, (b) adult fibroblasts with and without nucleofection of SOKM, and ( c ) CD34+ cord blood cells at various time points during induction of pluripotency with SOKM, with or without co-culture with bone marrow stromal cells (BMSC).
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| Overall design |
Total RNA was harvested from adult fibroblasts, adult fibroblasts three days after nucleofection with 4F (SOKM), Day -3 unstimulated cord blood (CB) cells, Day 0 growth factor stimulated CB cells, Day +3 CB cells with or without nucleofection at Day zero and with or without Day zero to Day +3 co-culture with bone marrow stromal cells (BMSC), Day 23 CB cells nucleofected on day zero and co-cultured on BMSC from Day Zero to Day +3, and undifferentiated H9 human embryonic stem cells. All experimental conditions repeated in three independent trials, and each replicate at each condition analyzed on an individual microarray.
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| Contributor(s) |
Park TS, Huo JS, Peters A, Talbot CC, Kaplan IM, Zambidis ET |
| Citation(s) |
22905176 |
| Submission date |
Jan 11, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Elias Thomas Zambidis |
| Organization name |
Johns Hopkins School of Medicine
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| Department |
Oncology
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| Street address |
733 N. Broadway
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (30)
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| GSM860964 |
Cord Blood Day 23 +4F +BMSC, Experiment 1 |
| GSM860965 |
Cord Blood Day 23 +4F +BMSC, Experiment 2 |
| GSM860966 |
Cord Blood Day 23 +4F +BMSC, Experiment 3 |
| GSM860967 |
Cord Blood Day -3, Experiment 1 |
| GSM860968 |
Cord Blood Day zero, Experiment 1 |
| GSM860969 |
Cord Blood Day +3 no 4F, no BMSC, Experiment 1 |
| GSM860970 |
Cord Blood Day +3 no 4F, +BMSC, Experiment 1 |
| GSM860971 |
Cord Blood Day +3 +4F, no BMSC, Experiment 1 |
| GSM860972 |
Cord Blood Day +3 +4F, +BMSC, Experiment 1 |
| GSM860973 |
Cord Blood Day -3, Experiment 2 |
| GSM860974 |
Cord Blood Day zero, Experiment 2 |
| GSM860975 |
Cord Blood Day +3 no 4F, no BMSC, Experiment 2 |
| GSM860976 |
Cord Blood Day +3 no 4F, +BMSC, Experiment 2 |
| GSM860977 |
Cord Blood Day +3 +4F, no BMSC, Experiment 2 |
| GSM860978 |
Cord Blood Day +3 +4F, +BMSC, Experiment 2 |
| GSM860979 |
Fibroblast Day+3 +4F Experiment 1 |
| GSM860980 |
Fibroblast Day+3 +4F Experiment 2 |
| GSM860981 |
Fibroblast Day+3 +4F Experiment 3 |
| GSM860982 |
Fibroblast Day zero, Experiment 1 |
| GSM860983 |
Fibroblast Day zero, Experiment 2 |
| GSM860984 |
Fibroblast Day zero, Experiment 3 |
| GSM860985 |
Cord Blood Day -3, Experiment 3 |
| GSM860986 |
Cord Blood Day zero, Experiment 3 |
| GSM860987 |
Cord Blood Day +3 no 4F, no BMSC, Experiment 3 |
| GSM860988 |
Cord Blood Day +3 no 4F, +BMSC, Experiment 3 |
| GSM860989 |
Cord Blood Day +3 +4F, no BMSC, Experiment 3 |
| GSM860990 |
Cord Blood Day +3 +4F, +BMSC, Experiment 3 |
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| This SubSeries is part of SuperSeries: |
| GSE35029 |
Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate iPSC reprogramming of lineage-committed myeloid progenitors |
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| Relations |
| BioProject |
PRJNA156183 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35027_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE35027_non-normalized.txt.gz |
16.1 Mb |
(ftp)(http) |
TXT |
| GSE35027_normalized.txt.gz |
4.0 Mb |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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