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Series GSE35010 Query DataSets for GSE35010
Status Public on Sep 05, 2012
Title Expression data from human hematopoietic stem and progenitor compartments from patients with acute myeloid leukemia with monosomy 7 and healthy controls
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We applied a novel approach of parallel transcriptional analysis of multiple, highly fractionated stem and progenitor populations in a genetically defined subset of AML (AML with monosomy 7). We isolated phenotypic long-term HSC (LT-HSC), short-term HSC (ST-HSC), and committed granulocyte-monocyte progenitors (GMP) from individual patients with AML, and measured gene expression profiles of each population, and in comparison to their phenotypic counterparts from age-matched healthy controls.
 
Overall design Bone marrow samples from AML patients bearing monosomy 7 and age-matched healthy controls were used in this study. Hematopoietic stem and progenitor compartments were purified by multiparameter-high speed fluorescence-activated cell sorting (FACS) from CD34+ enriched bone marrow to isolate LT-HSC (Lin-/CD34+/CD38-/CD90+), ST-HSC (Lin-/CD34+/CD38-/CD90-), and GMP (Lin-/CD34+/CD38+/CD123+/CD45R+).
 
Contributor(s) Barreyro L, Steidl U
Citation(s) 22723552, 25810490
Submission date Jan 11, 2012
Last update date Jul 26, 2018
Contact name Boris Bartholdy
Organization name Albert Einstein College of Medicine
Department Cell Biology
Street address 1300 Morris Park Ave
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (31)
GSM860584 short term hematopoietic stem cell_healthy control_C0109N
GSM860585 short term hematopoietic stem cell_healthy control_C0108N
GSM860586 short term hematopoietic stem cell_-7AML_10120A
Relations
BioProject PRJNA151165

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Supplementary file Size Download File type/resource
GSE35010_RAW.tar 117.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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