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Series GSE34934 Query DataSets for GSE34934
Status Public on Jan 10, 2012
Title Expression data from BDC2.5 TCR Tg, preselected Rag-/-.B6 and Rag-/-.NOD.H2b thymocytes upon antigenic stimulation
Organism Mus musculus
Experiment type Expression profiling by array
Summary The aim of this study was to quantify the impact of NOD genetic vatiation on thymic negative selection transcriptional programs.
 
Overall design Pre-selected BDC2.5 TCR Tg DP thymocytes from non-selecting B6 and NOD.H2b backgrounds were purified (Dynal CD8 FlowComp), mixed in a 1:1 ratio and stimulated with BDC mimotope-loaded TCRa-/-/NOD splenocytes for indicated periods of time and double sorted by FACS as Thy1.2+Dump-CD4+CD8+; Dump includes CD19, Gr1, CD11b, CD11c, CD49b. Following cell sorting into trizol, RNA was purified, labeled and hybridized to Affymetrix arrays.
experiment type: unstimulated versus stimulated BDC/B6.Rag-/- and BDC/NOD.H2b.Rag-/- DP thymocytes
 
Contributor(s) Mingueneau M, Benoist C, Mathis D
Citation(s) 22329992
Submission date Jan 09, 2012
Last update date Mar 04, 2019
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (43)
GSM857789 BDC/B6 DP 3h CTR replicate 1
GSM857790 BDC/B6 DP 3h CTR replicate 2
GSM857791 BDC/B6 DP 7h CTR replicate 2
This SubSeries is part of SuperSeries:
GSE34936 NOD genetic variation influences ab/gd lineage decisions when TCRa is prematurely expressed, but not the process of negative selection.
Relations
BioProject PRJNA156147

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34934_RAW.tar 170.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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