|
| Status |
Public on Nov 22, 2012 |
| Title |
Dual knockdown of Esrrb and Sox2 in mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Esrrb, Sox2 or Esrrb and Sox2 were knocked down in mouse ES cells using shRNA plasmid pSUPER.puro containing shRNAs from the publications Feng et al., 2009 and Rodda et al., 2005.
Knockdown was transfected into mouse ES cells using lipofectamine and then cells were selected for knockdown using puromycin.
RNA was harvested after 2 days of knockdown.
|
| |
|
| Overall design |
Esrrb and Sox2 were knocked down in mouse ES cells either individually or together, cells were selected with puromycin and RNA harvested and subjected to microarray after 2 days.
|
| |
|
| Contributor(s) |
Robson P, Hutchins AP |
| Citation(s) |
23169531 |
| Submission date |
Dec 05, 2011 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Andrew P Hutchins |
| E-mail(s) |
andrewh@sustech.edu.cn
|
| Organization name |
Southern University of Science and Technology
|
| Department |
Bioinformatics
|
| Lab |
Bioinformatics and Genomics
|
| Street address |
1088 Xueyuan Rd
|
| City |
Shenzhen |
| ZIP/Postal code |
518055 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (4)
|
| GSM843387 |
ES cells Esrrb&Sox2 shRNA rep1-rep4 |
|
| Relations |
| BioProject |
PRJNA149721 |
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