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| Status |
Public on Jan 01, 2013 |
| Title |
Expression data from mesodermal Tbx1-knockout ears at E12.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The T-box transcription factor Tbx1 is expressed in the otic vesicle and surrounding periotic mesenchyme during inner ear development. Mesenchymal Tbx1 is essential for inner ear development, with conditional mutants displaying defects in both auditory and vestibular systems. We have previously identified reduced expression of retinoic acid metabolic genes in the periotic mesenchyme of mesoderm conditional Tbx1 mutants, using the T-Cre mouse line, implicating retinoic acid in mesenchymal-epithelial signaling downstream of Tbx1 in the periotic mesenchyme.
In order to identify downstream effectors of mesenchymal-epithelial signaling downstream of mesenchymal Tbx1, we have utilized a gene profiling approach comparing embryonic day 12.5 periotic tissue from T-Cre-mediated conditional Tbx1 mutants (Mest-KO) and conditional heterozygous control litter mates (control).
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| Overall design |
E12.5 T-Cre-mediated conditional Tbx1 mutants (Mest-KO) and control (T-Cre conditional Tbx1 heterozygotes) embryos were microdissected to isolate the otic vesicle and surrounding periotic mesenchyme. Left and right tissue from each individual embryo was pooled, and 5 control and 5 mutant embryos were analyzed on individual microarrays using Affymetrix Mouse Gene ST 1.0 chips.
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| Contributor(s) |
Monks DC, Morrow BE |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 30, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Tingwei Guo |
| E-mail(s) |
tingwei.guo@einstein.yu.edu
|
| Phone |
(718) 678-1122
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Genetics
|
| Lab |
Morrow Lab
|
| Street address |
1301 Morris Park Avenue, Room 408
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| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
|
| GSM841177 |
T-Cre conditional Tbx1 knockout periotic tissue at E12.5, biological replicate 1 |
| GSM841178 |
T-Cre conditional Tbx1 knockout periotic tissue at E12.5, biological replicate 2 |
| GSM841179 |
T-Cre conditional Tbx1 knockout periotic tissue at E12.5, biological replicate 3 |
| GSM841180 |
T-Cre conditional Tbx1 knockout periotic tissue at E12.5, biological replicate 4 |
| GSM841181 |
T-Cre conditional Tbx1 knockout periotic tissue at E12.5, biological replicate 5 |
| GSM841182 |
T-Cre conditional Tbx1 heterozygous control periotic tissue at E12.5, biological replicate 1 |
| GSM841183 |
T-Cre conditional Tbx1 heterozygous control periotic tissue at E12.5, biological replicate 2 |
| GSM841184 |
T-Cre conditional Tbx1 heterozygous control periotic tissue at E12.5, biological replicate 3 |
| GSM841185 |
T-Cre conditional Tbx1 heterozygous control periotic tissue at E12.5, biological replicate 4 |
| GSM841186 |
T-Cre conditional Tbx1 heterozygous control periotic tissue at E12.5, biological replicate 5 |
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| This SubSeries is part of SuperSeries: |
| GSE34066 |
Expression data from mesodermal Tbx1-knockout ears, otic vesicles, and periotic mesenchyme |
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| Relations |
| BioProject |
PRJNA156509 |
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