|
| Status |
Public on Oct 15, 2012 |
| Title |
Vastus lateralis muscle gene expression after local physical deconditioning and exercise training |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The leg of healthy volunteers was locally deconditioned using three weeks of unilateral lower limb suspension (ULLS). The extremely deconditioned legs of subjects with a spinal cord injury (SCI) were trained using eight weeks of functional electrical stimulation (FES) exercise, 2-3 times per week (total 20 sessions).
These models for local (in)activity were used to study changes in gene expression level of: A) Long-term deconditioning: before FES versus before ULLS (=SCI versus control); B) Short-term deconditioning: after ULLS versus before ULLS; and C) Exercise training: after FES versus before FES.
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| |
|
| Overall design |
RNA was isolated from six healthy subjects before and after ULLS deconditioning, and three subjects with SCI before and after FES exercise training.
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| |
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| Contributor(s) |
vanDuijnhoven NT, Lammers G, Hopman MT |
| Citation(s) |
23011062, 23064509 |
| Submission date |
Nov 22, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Gerwen Lammers |
| E-mail(s) |
gerwenlammers@gmail.com
|
| Organization name |
Radboud University Nijmegen Medical Centre
|
| Street address |
PO Box 9101
|
| City |
Nijmegen |
| ZIP/Postal code |
6500 HB |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (14)
|
| GSM838371 |
Healthy subject 1 before limb suspension |
| GSM838372 |
Healthy subject 1 after limb suspension |
| GSM838373 |
Healthy subject 2 before limb suspension |
| GSM838374 |
Healthy subject 2 after limb suspension |
| GSM838375 |
Healthy subject 3 before limb suspension |
| GSM838376 |
Healthy subject 3 after limb suspension |
| GSM838377 |
Healthy subjects 4, 5, and 6 (pooled RNA) before limb suspension |
| GSM838378 |
Healthy subjects 4, 5, and 6 (pooled RNA) after limb suspension |
| GSM838379 |
SCI subject 1 before FES exercise training |
| GSM838380 |
SCI subject 1 after FES exercise training |
| GSM838381 |
SCI subject 2 before FES exercise training |
| GSM838382 |
SCI subject 2 after FES exercise training |
| GSM838383 |
SCI subject 3 before FES exercise training |
| GSM838384 |
SCI subject 3 after FES exercise training |
|
| Relations |
| BioProject |
PRJNA148319 |
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