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| Status |
Public on Nov 15, 2011 |
| Title |
Expression data of NCI-H441 cells stably expressing hsa-mir-365-2 vs empty vector |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Hsa-mir-365-2 is one of the two precursors that give rise to miR-365. We discovered that miR-365 directly regulates a lung cancer and developmental gene termed thyroid transcription factor 1 (TTF-1 or NKX2-1).
Hsa-mir-365-2 was transfected into NCI-H441 cells via retrovirus-mediated gene transfer. Arrays hybridized: Affymetrix GeneChip Human Gene 1.0 ST Array.
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| Overall design |
A total of 4 samples were analyzed. The first two were H441 cells expressing hsa-mir-365-2 and the other two were H441 transfected with empty vector. The class comparison function (significant cutoff, 0.001) of BRB Array Tool (4.1.0 beta_3 release) was used to analyze for differentially expressed genes as a result of enforced expression of hsa-mir-365-2.
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| Contributor(s) |
Qi J, Mu D |
| Citation(s) |
22185756 |
| Submission date |
Nov 14, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
David Mu |
| Organization name |
Penn State Universitiy College of Medicine
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| Street address |
500 University Dr H083
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| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| GSM832614 |
mir-365-2 expressor, biological rep 1 |
| GSM832615 |
mir-365-2 expressor, biological rep 2 |
| GSM832616 |
empty-vector expressor, biological rep 1 |
| GSM832617 |
empty-vector expressor, biological rep 2 |
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| Relations |
| BioProject |
PRJNA148717 |
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