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| Status |
Public on Oct 18, 2011 |
| Title |
Gene expression profiles of DLD1 cells in which GATA6 or LGR5 expression was suppressed. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Mutations in APC or β-catenin that cause aberrant activation of Wnt signaling are responsible for the initiation of colorectal tumor development. LGR5 is specifically expressed in stem cells of the intestine, stomach and hair follicle, and plays essential roles in maintaining tissue homeostasis. LGR5-positive stem cells have been shown to be responsible for the intestinal adenoma initiated by some mutations in APC . Furthermore, it has recently been reported that Lgr5, which is associated with the Frizzled/Lrp Wnt receptor complex, interacts with R-spondins and thereby activates Wnt signaling. However, the function of LGR5 in colorectal tumorigenesis has been unclear. Here we show that LGR5 is required for the tumorigenicity of colorectal cancer cells. We also show that the transcription factor GATA6 directly enhances the expression of LGR5.
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| Overall design |
DLD1 cells were infected with a lentivirus expressing an shRNA targeting GATA6 or LGR5.
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| Contributor(s) |
Taniue K, Hayashi T, Tsuji S, Kawasaki Y, Akiyama T |
| Citation(s) |
24452072 |
| Submission date |
Oct 14, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Tetsu Akiyama |
| E-mail(s) |
akiyama@iam.u-tokyo.ac.jp
|
| Organization name |
Tokyo University
|
| Department |
Institute of Molecular and Cellular Biosciences
|
| Lab |
Laboratory of Molecular and Genetic Information
|
| Street address |
1-1-1, Yayoi, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
110-0032 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA146445 |
