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| Status |
Public on Aug 31, 2014 |
| Title |
The genes associated with the response to S-1,cisplatin and docetaxel combination chemotherapy (DCS therapy) for metastatic gastric cancer: |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Despite the effort in defining the molecular mechanisms for the drug resistance, predictors of response to chemotherapy have yet to be developed in gastric cancer.
With microarray of whole human genes, we determined 29 genes associated with the response to cisplatin and docetaxel combination chemotherapy for metastatic gastric cancer.
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| Overall design |
We obtained Fresh-frozen samples of tumor tissue and background gastric mucosa tissue from 19 patients with gastric cancer by endoscopic biopsy before DCS therapy. Total RNA was extracted from individual microdissected populations of cancer cells and background mucosa cells using RNAeasy mini kits and amplified with a random primer, WT-Ovation FFPE RNA Amplification System V2; NuGEN, Cincinnati, Ohio) according to the manufacturer’s protocols. The amplified fragmented RNA was hybridized on a Whole Human Genome Oligo Microarray Chip (Agilent Technologies) containing 44,000 cDNA clones.
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| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 01, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Shinji Kitamura |
| Organization name |
tokushima university
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| Street address |
kuramotocho 3-18-15
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| City |
tokushima city |
| ZIP/Postal code |
770-8503 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (38)
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| Relations |
| BioProject |
PRJNA145249 |
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