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| Status |
Public on Dec 24, 2011 |
| Title |
Integration of BRCA1-mediated miRNA and mRNA signatures reveal miR-146a, miR-99b and miR-205 regulation of the TRAF2 and NFkB pathways (mRNA dataset) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
BRCA1 deregulation is a frequent event in the pathogenesis of breast as well as other cancers. In addition to the DNA repair functions of BRCA1, it is involved in a wide range of cellular processes such as cell cycle, chromatin remodeling or transcription. However, the molecular events underlying BRCA1-associated tumorigenesis are still largely unknown. In order to deepen our understanding of BRCA1-associated tumorigenesis, we integrated data from mRNA and miRNA microarray experiments on the HCC1937 breast cancer cell line, and the isogenic HCC1937 stably expressing BRCA1, to obtain significant miRNA-mRNA relationships associated to the presence of the BRCA1 gene. Our results demonstrate that integration of mRNA and miRNA associated to BRCA1 expression was useful to discover new miRNA-gene interactions as molecular events underlying BRCA1-mediated tumorigenesis.
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| |
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| Overall design |
Transcriptional profiling of the BRCA1-null HCC1937 cell line and HCC1937 cells after stable transfection of BRCA1.
Two-condition experiment: Universal Human Reference RNA (Stratagene, catalog #740000) (Cy3) vs. cell line (Cy5). Biological replicates: 3 HCC1937, 3 BRCA1-transfected HCC1937 cells.
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| |
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| Contributor(s) |
Tanic M, Zajac M, Gómez-López G, Benítez J, Martínez-Delgado B |
| Citation(s) |
22167321 |
| Submission date |
Jul 20, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Beatriz Martinez |
| E-mail(s) |
bmartinez@cnio.es
|
| Organization name |
Spanish National Cancer Research Centre (CNIO)
|
| Department |
Human Cancer Genetics
|
| Street address |
Calle Melchor Fernandez Almagro 3
|
| City |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE30822 |
Integration of BRCA1-mediated miRNA and mRNA signatures reveal miR-146a, miR-99b and miR-205 regulation of the TRAF2 and NFkB pathways |
|
| Relations |
| BioProject |
PRJNA154567 |
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