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| Status |
Public on Jul 15, 2011 |
| Title |
Sox17-transgenic hematopoietic stem cell microarray |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The transcription factor SOX17 is expressed by fetal, but not adult hematoipoietic stem cells (HSCs), and is required for the maintenance of fetal and neonatal, but not adult, HSCs. In the current study we show that ectopic expression of Sox17 in adult HSCs and transiently reconstituting multipotent progenitors was sufficient to confer increased self-renewal potential and the expression of fetal HSC genes including fetal HSC surface markers.
To assess the acute effects of ectopic Sox17 expression on global gene expression in adult HSCs, we performed microarray analysis to compare the gene expression profile of adult Sox17-trangenic and control HSCs after short induction of Sox17-transgene expression.
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| Overall design |
Total RNA were isolated from 5 independent, freshly isolated aliquots of 10,000 HSCs isolated from 8-week old Sox17-transgenic ((tetO)7CMVSox17-IRES-NucEGFP;B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J double transgenic) or littermate control mice that were treated with doxycycline for 5 days to induce transgene expression. Purified RNA was reverse transcribed and amplified using the WT-Ovation™ Pico RNA Amplification system (NuGEN Technologies) following the manufacturer’s instructions. Sense strand cDNA was generated using WT-Ovation™ Exon Module (NuGEN), then fragmented and labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN). 2.5µg of labeled cDNA were hybridized to Affymetrix Mouse Gene ST 1.0 microarrays.
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| Contributor(s) |
He S, Morrison SJ |
| Citation(s) |
21828271 |
| Submission date |
Jul 06, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Shenghui He |
| E-mail(s) |
hesh@umich.edu
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| Organization name |
University of Michigan
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| Department |
Life Science institute
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| Lab |
Sean Morrison
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| Street address |
210 Washtenaw Avenue, 5258 LSI
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| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE30446 |
Transcription factor SOX17 overexpression in hematopoietic stem cells |
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| Relations |
| BioProject |
PRJNA154741 |
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