|
| Status |
Public on Jul 07, 2011 |
| Title |
Identification of right heart-enriched genes in a murine model of chronic outflow tract obstruction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The right ventricle (RV) differs in several aspects from the left ventricle (LV) including its embryonic origin, physiological role and anatomical design. In contrast to LV hypertrophy, little is known about the molecular circuits, which are activated upon RV hypertrophy (RVH). We established a highly reproducible model of RVH in mice using pulmonary artery clipping (PAC), which avoids detrimental RV pressure overload and thus allows long-term survival of operated mice. Magnetic resonance imaging revealed pathognomonic changes with striking similarities to human congenital heart disease- or pulmonary arterial hypertension- patients. Comparative, microarray based transcriptome analysis of right- and left-ventricular remodeling identified distinct transcriptional responses to pressure-induced hypertrophy of either ventricle, which were mainly characterized by stronger transcriptional responses of the RV compared to the LV myocardium. Hierarchic cluster analysis revealed a RV- and LV-specific pattern of gene activity after induction of hypertrophy, however, we did not find evidence for qualitatively distinct regulatory pathways in RV compared to LV. Data mining of nearly three thousand RV-enriched genes under PAC disclosed novel potential (co)-regulators of long-term RV remodeling and hypertrophy. We reason that specific inhibitory mechanisms in RV restrict excessive myocardial hypertrophy and thereby contribute to its vulnerability to pressure overload.
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| Overall design |
Alternative splicing and gene expression analysis during development of the heart and cardiomyoyte differentiation.
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| Contributor(s) |
Kreymborg Kg, Uchida S, Gellert P, Schneider A, Boettger T, Voswinckel R, Wietelmann A, Szibor M, Weissmann N, Ghofrani AH, Schermuly R, Schranz D, Seeger W, Braun T |
| Citation(s) |
20673770 |
| Submission date |
Jul 06, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Pascal Gellert |
| E-mail(s) |
pascal.gellert@mpi-bn.mpg.de
|
| Phone |
+49 6032 705 1765
|
| URL |
http://www.mpi-hlr.de
|
| Organization name |
Max Planck Institut for Heart and Lung Research
|
| Department |
Cardiac Development and Remodelling
|
| Lab |
Braun
|
| Street address |
Ludwigstr. 43
|
| City |
Bad Nauheim |
| ZIP/Postal code |
61231 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (12)
|
| GSM754729 |
Transaortic constriction left ventricle_3wk_ 1 |
| GSM754730 |
Transaortic constriction left ventricle_3wk_ 2 |
| GSM754731 |
sham left ventricle_3wk_1 |
| GSM754732 |
sham left ventricle_3wk_2 |
| GSM754733 |
pulmonary artery clipping right ventricle_1wk_1 |
| GSM754734 |
pulmonary artery clipping right ventricle_1wk_2 |
| GSM754735 |
pulmonary artery clipping right ventricle_3wk_1 |
| GSM754736 |
pulmonary artery clipping right ventricle_3wk_2 |
| GSM754737 |
pulmonary artery clipping right ventricle_6wk_1 |
| GSM754738 |
pulmonary artery clipping right ventricle_6wk_2 |
| GSM754739 |
sham right ventricle_3wk_1 |
| GSM754740 |
sham right ventricle_3wk_2 |
|
| Relations |
| BioProject |
PRJNA143529 |
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