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| Status |
Public on May 16, 2011 |
| Title |
Gene expression profiles after knockdown of Nardilysin in TMK-1 gastric cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Nardilysin (NRDc), a metalloendopeptidase of the M16 family, has been reported to promote the ectodomain shedding and resulting activation of various growth factors and cytokines, but its role in cancer biology have not been elucidated.
Microarray experiments were performed to analyze the chnages of gene expression profiles after knockdown of NRDc by microRNA (miR)-based RNA interference (RNAi).
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| Overall design |
RNAi experiments were perfomed using BLOCK-iT Pol II miR RNAi Expression Vector Kit with EmGFP (Invitrogen). TMK-1 cells stably expressing conrol or NRDc-targeting miR vector were established.
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| Contributor(s) |
Kanda K, Komekado H, Seno H, Nishi E |
| Citation(s) |
22351606 |
| Submission date |
May 09, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Hideyuki Komekado |
| E-mail(s) |
komekado@kuhp.kyoto-u.ac.jp
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| Phone |
+81-75-751-4319
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| Fax |
+81-75-751-4303
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| Organization name |
Graduate School of Medicine, Kyoto University
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| Department |
Gastroenterology and Hepatology
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| Street address |
54 Kawara-cho, Shogoin, Sakyo-ku,
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| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
| GSM721515 |
TMK-1 cells stably expressing miR-negative control clone #4 |
| GSM721516 |
TMK-1 cells stably expressing miR-NRDc clone #1 |
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| Relations |
| BioProject |
PRJNA140349 |
