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| Status |
Public on Apr 01, 2017 |
| Title |
Identification of GLI1 direct and indirect target genes in human prostate cancer cell line PC3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
PC3GLI1ER and PC3ER were established from human prostate cancer cell line PC3. PC3GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, PC3ER harbor only EGFP-tagged AF2 domain.
To identify GLI1 direct target genes, total RNAs were purified from the cells before and 24 hours after the beta-estradiol (E2) treatment.
Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray.
Keywords: Expression profiling by array
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| Overall design |
GLI1 direct and indirect target genes were identified in PC3 cells. Gene expression profiles of PC3GLI1ER and PC3ER cells were analyzed by AGILENT human 4x44 cDNA microarray before and 24 hours after treatment of beta-estradiol (E2) at doses of 10nM.
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| Contributor(s) |
Inaguma S, Kasai K, Ikeda H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 28, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Shingo Inaguma |
| Organization name |
Aichi Medical University School of Medicine
|
| Department |
Department of Pathology and Promoting Center for Clinical Research
|
| Street address |
Nagakute
|
| City |
Aichi |
| ZIP/Postal code |
480-1195 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (4)
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| GSM712019 |
PC3GLI1ER_10nM beta-estradiol (E2)_24hrs |
|
| Relations |
| BioProject |
PRJNA140657 |
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