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| Status |
Public on Mar 18, 2011 |
| Title |
Aldosterone-inducible genes in mineralocorticoid receptor expressed human endothelial cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To elucidate the aldosterone-regulated genes in vascular endothelial cells, we developed human endothelial cell line (EAhy926) stably expressing human mineralocorticoid receptor by retroviral system (MR-EAhy). Then gene expression profile of MR-EAhy stimulated with or without aldosterone was compared using DNA microarray analysis.
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| Overall design |
MR-EAhy were serum-deprived for 24h with 0.2% dextran-coated charcoal-treated FBS, and then stimulated with or without aldosterone (10-9 M) for 6 hr. Then cells were harvested for RNA extraction.
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| Contributor(s) |
Yoshimoto T, Sekizawa N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 17, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Takanobu Yoshimoto |
| Organization name |
Tokyo Medical and Dental University Graduate School
|
| Department |
Department of Clinical and Molecular Endocrinology
|
| Street address |
1-5-45 Yushima, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8510 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (6)
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| GSM692751 |
InVitroCells_6h_Aldosterone10-9M_rep1 |
| GSM692752 |
InVitroCells_6h_Aldosterone10-9M_rep2 |
| GSM692753 |
InVitroCells_6h_Aldosterone10-9M_rep3 |
|
| Relations |
| BioProject |
PRJNA137983 |
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